Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 试验地点
- 2
- 主要终点
- Annexin A 5 targeting in the non dominant thenar muscle after (ischemic exercise), as a indicator for ischemia reperfusion injury.
研究概览
简要总结
To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients.
To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.
详细描述
Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Age 18-50 years
- •No physical limitation to perform ischemic exercise
- •Informed consent
排除标准
- •Diabetes (fasting glucose > 7,0mmol/l, or random glucose >11,0mmol/l)
- •hyperlipidemia (random total cholesterol > 6.5 mmol/l)
- •Hypertension (supine SBP/DBP > 140/90 mmHg at screening)
- •Any cardiovascular disease
- •Drug abuse
- •Concomitant chronic use of medication
- •Administration of radioactivity in research setting during the last 5 years
- •Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.
结局指标
主要结局
Annexin A 5 targeting in the non dominant thenar muscle after (ischemic exercise), as a indicator for ischemia reperfusion injury.
次要结局
- Workload during (ischemic) exercise.