Acute Effects of Beetroot Juice and Caffeine Co-ingestion on Lower-limb Strength and Muscular Endurance in Females

Not Applicable

Completed

• Conditions: Beetroot Juice; Caffeine; Placebo; Beetroot Juice with Caffeine Co-ingestion

• Registration Number: NCT06758323
• Lead Sponsor: Jose Manuel Jurado Castro

Brief Summary

The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are:

* Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo?

* How do the acute effects of beetroot juice interact with caffeine on physical performance?

The study was conducted using a randomized, crossover design to evaluate the following four conditions:

Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only

Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance.

Participant Protocol

Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.

Detailed Description

Not available

Study Timeline

• Study Start: 2024-08-01
• Primary Completion: 2024-12-26
• Study Completion: 2024-12-26
• Study First Submitted: 2024-12-26
• Study First Submitted QC: 2024-12-26
• Status Verified: 2025-01
• Study First Posted: 2025-01-03
• Last Update Submitted: 2025-01-03
• Last Update Posted: 2025-01-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 15

Inclusion Criteria

• Minimal experience of six months of strength training
• Be familiarized with the exercise test: counter movement jump (CMJ), barbell back squats, leg press and knee extension.

Exclusion Criteria

• Supplementation consumption
• Sports injuries

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Change of height reached in jump	Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)	Evaluated through Countermovement jump test with a recovery period of 45 seconds between jumps. The height reached was recorded in centimeters.

Secondary Outcome Measures

Name	Time	Method
Movement velocity	Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)	The velocity was recorded in meters per second (m/s) during two repetitions of the back squat, evaluated at 50% and 75% of the repetition maximum (RM).
Muscular endurance test	Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)	The test consists of three exercises: back squat, leg press and leg extension. Three series will be performed until concentric failure at 75% RM, three minutes of rest between each series (3 series). The number of repetitions was recorded.
Rating of perceived exertion (RPE).	Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)	Immediately at the end of the muscular endurance test, participants will be instructed to report their RPE using a scale 1-10 (worse to better outcome).

Trial Locations

Locations (1)

Edificio IMIBIC Avda Menéndez Pidal s/n; 🇪🇸 Córdoba, Spain