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Effect of Glycogen Replenishment on Time Trial Performance Following a Glycogen Lowering Exercise

Not Applicable
Conditions
Carbohydrate Metabolism
Interventions
Behavioral: 20 km time trial performance
Behavioral: Glycogen lowering exercise
Registration Number
NCT03682861
Lead Sponsor
Western University, Canada
Brief Summary

Fifteen endurance-trained male/female will be randomly assigned to do four exercise and nutrition trials involving ingestion of four different concentrations of sweet corn derived starch (food component) in water (0, 1, 1.5 and 2 g. kg-1. h-1). Each trial will be separated by at least one week. During these four experimental trials athletes will be subjected to a glycogen-lowering cycling exercise protocol followed by a 4-h post-exercise recovery period (2h feeding then 2 hours of rest). At the end of 4-h period they will do a 20 kilometre time trial test on a stationary bike in a laboratory condition to measure the effect of different glycogen repletion rates on exercise performance.

Detailed Description

Post-exercise glycogen synthesis rate is an important factor in determining the time needed to recover. Glycogen synthesis is affected not only by the extent of glycogen depletion but also in a more direct manner by the type, duration, and intensity of the preceding exercise because these will differentially influence the acute enzymatic changes as well as recovery from the acute changes that are induced by strenuous exercise. To optimize glycogen synthesis rates, adequate amounts of carbohydrate should be ingested. It has been suggested initially that a carbohydrate intake of 0.35 g·kg body wt-1 ·h-1 , provided at 2-h intervals, maximized muscle glycogen synthesis. Others observed no differences in glycogen storage rates after subjects ingested 0.75 or 1.5 g carbohydrate·kg-1 · h-1 provided at 2-h intervals. In a follow-up study, it was reported that an intake of \>0.5 g·kg-1 · h-1 is necessary to maximize post-exercise glycogen synthesis if supplements are administered at 2-h intervals. Higher glycogen synthesis rates have been reported in studies in which carbohydrates were ingested more frequently and at higher ingestion rates than in previous studies. Other efforts to increase glycogen synthesis rates by changing the form of administration (ie, as a solution, as a solid, or intravenously) have been unsuccessful. While the above range of intake rates has been suggested to maximize muscle glycogen resynthesis post-exercise, the required dosage with sweet corn derived high glycemic starch and its effect on a subsequent time trial exercise is currently unknown. So, investigators objective in this experiment is to find out optimal sweet corn derived recovery ingestion dose by using a glycogen lowering exercise protocol, followed by glycogen repletion via ingestion and a subsequent 20 km time trial cycling performance.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
15
Inclusion Criteria
  • are a healthy male or female endurance athlete of 18- 40 years of age
  • have ≥2 y endurance training experience; training for ˃1 h/day
Exclusion Criteria
  • Have symptoms or take medication for respiratory disease
  • Have symptoms or take medication for cardiovascular disease
  • Have symptoms or take medication for metabolic disease
  • Have symptoms or take mediation for neuromuscular disease
  • Use heart rate or blood pressure medications
  • Use any medications with side effects of dizziness, lack of motor control, or slowed reaction time
  • Have any cardiovascular or neuromuscular limitations to exercise
  • Are pregnant or find out that you are pregnant during experiment

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Placebo20 km time trial performancePlacebo flavored drink similar to treatments but with no energy will be ingested post glycogen lowering exercise, followed by a 20 km time trial intervention
Carbohydrate drinksGlycogen lowering exerciseSweet corn derived starch mixed in water at three different concentrations (6%, 12% and 18%) will be ingested post glycogen lowering exercise, followed by a 20 km time trial intervention
Carbohydrate drinks20 km time trial performanceSweet corn derived starch mixed in water at three different concentrations (6%, 12% and 18%) will be ingested post glycogen lowering exercise, followed by a 20 km time trial intervention
PlaceboGlycogen lowering exercisePlacebo flavored drink similar to treatments but with no energy will be ingested post glycogen lowering exercise, followed by a 20 km time trial intervention
Primary Outcome Measures
NameTimeMethod
20 km time trial40 minutes

participants will ride for 20 km on a stationary bike and time to finish will be measured

Secondary Outcome Measures
NameTimeMethod
VO22 hours

VO2 will be measured using a metabolic cart

Blood glucose2 hours

glucose will be measured using glucometer

VCO22 hours

VCO2 will be measured using a metabolic cart

Serum insulin2 hours

Insulin will be measured using an immumoassay kit

Blood lactate2 hours

blood lactate will be measured using lactate meter

Trial Locations

Locations (1)

Exercise Nutrition Laboratory (Western University)

🇨🇦

London, Ontario, Canada

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