Vascular Health With Immobilization and Exercise Training
- Conditions
- Physical ActivityImmobilization, Tonic
- Interventions
- Other: ImmobilizationOther: Training with non immobilized legOther: Exercise training with both legs
- Registration Number
- NCT03054376
- Lead Sponsor
- University of Copenhagen
- Brief Summary
The study includes healthy young habitually active subjects. All subjects are subjected to two weeks of immobilization of one leg followed by four weeks of training of both legs. The subjects are randomized to either one-leg training of the non-immobilized leg during the two-week immobilization period or to no training of the non-immobilized leg during the immobilization period. Measures include skeletal muscle morphology, markers of angiogenesis and apoptosis, muscle metabolic markers, vascular function, vascular proteins and performance.
- Detailed Description
One leg of young healthy subjects will be immoblized for two weeks. Changes in angiogenic proteins and markers of apoptosis in muscle tissue and muscle extracellular fluid will be determined in both legs and in parallel the functional consequences of immobilization will be assessed. Vascular function and vascular proteins will also be determined. In order to understand how the oxidative capacity of the muscle is altered in parallel with changes in capillarization, markers of mitochondria will be measured .Half of the subjects will be randomized to single-leg training during the immobilization period to assess whether simultaneous training reduces the loss of capillaries, muscle function and vascular function in the immobilized leg. After the immobilization period the study participants will train the immobilized leg and the control leg for four weeks by moderate to intense interval cycle training and the same parameteres as during immobilization will be assessed in both legs. The study will also include measurements of circulating compounds and platelets in blood.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 12
- Healthy
- BMI <25
- Habitually/moderately physically active
- Known chronic medical disorder
- Smoking
- Excessive alcohol intake
- Known genetic/familial predisposition for thrombosis
- Hormonal birth control
- High level strength or endurance training on a regular basis
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Training of non-immobilized leg Training with non immobilized leg After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will be trained. After the two weeks the subjects will train both legs for four weeks. Training of non-immobilized leg Immobilization After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will be trained. After the two weeks the subjects will train both legs for four weeks. Training of non-immobilized leg Exercise training with both legs After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will be trained. After the two weeks the subjects will train both legs for four weeks. Immobilization Immobilization After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will not be trained. After the two weeks the subjects will train both legs for four weeks. Immobilization Exercise training with both legs After baseline studies, the subjects will have one leg immobilized by full length plaster for two weeks. During the two weeks, the other leg will not be trained. After the two weeks the subjects will train both legs for four weeks.
- Primary Outcome Measures
Name Time Method Angiogenesis Change from after immobilization to after 4 weeks of training Skeletal muscle capillarization-comparison between legs
Vascular function Change from after immobilization to after 4 weeks of training Measurements of skeletal muscle blood flow by ultrasound doppler in response to infusion of vasodilators/constrictors-comparison between legs
- Secondary Outcome Measures
Name Time Method Markers of bone metabolism Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measurements of bone markers in plasma
Knee extensor performance Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measurements of maximal power and endurance-comparison between legs
Vascular and metabolic proteins Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Proteins of importance for vascular function and muscle metabolism measured in muscle biopsy samples, skeletal muscle interstitial fluid and plasma-comparison between legs
Maximal oxygen uptake Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measured by gas analysis during an incremental ergometer cycle protocol
Body composition Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measurements by dual energy x-ray absorptiometry
Arterial blood pressure Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measured by sphygmomanometer and intra-arterially
Skin microcirculation Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measurements of skin blood flow and morphology and relevant proteins in skin biopsies
Markers of angiogenesis Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measurement of angiogenic and apoptotic factors-comparison between legs
Vascular function-non-invasive Change from baseline to after 2 weeks of immobilization and from after immobilization to after 4 weeks of training Measurements of skeletal muscle blood flow at rest, during passive movement, during exercise, after five min of occlusion and during infusion of vasodilators and constrictors-comparison between legs
Trial Locations
- Locations (1)
University of Copenhagen
🇩🇰Copenhagen, Denmark