Angiotensin-converting Enzyme 2 (ACE2) Expression in Tonsils and Adenoids

Withdrawn

• Conditions: Tonsillectomy; Adenoidectomy
• Interventions: Other: Storage of operating waste

• Registration Number: NCT04640610
• Lead Sponsor: Assistance Publique - Hôpitaux de Paris

Brief Summary

The objective of the research is to study the expression of the ACE 2 receptor and the TMPRSS2 serine protease in the tonsils and adenoids of children and adults.

Detailed Description

The entry point for SARS-CoV-2 into the body is the nasal and pharyngeal mucosa. Angiotensin-converting enzyme 2 (ACE 2) was quickly identified as the functional receptor for SARS-CoV-2. Furthermore, the TMPRSS2 serine protease is required for the anchoring of the S protein in cell lines. In addition, the most commonly used screening test is a test for viral RNA by RT-PCR on a nasopharyngeal sample because the viral load is high.

The tonsils and adenoids could be a target organ of the virus. In order to study this hypothesis, the expression of ACE 2 and the serine protease TMPRSS2 will be sought in these tissues.

In addition, children, generally at high risk of severe viral respiratory infections (RSV, influenza), have milder symptoms than older patients with SARS-CoV-2 infection. The reason for this difference remains elusive. But one of the hypotheses formulated is that the level of expression of ACE 2 and / or TMPRSS2 may differ between adults and children, the viral load seeming to be correlated with the severity of the attack. In order to study this hypothesis, we will therefore compare the level of expression of ACE 2 in the tonsil tissue of children and adults.

Study Timeline

• Study First Submitted: 2020-11-19
• Study First Submitted QC: 2020-11-19
• Study First Posted: 2020-11-23
• Status Verified: 2021-06
• Study Start: 2021-06-30
• Primary Completion: 2021-06-30
• Study Completion: 2021-06-30
• Last Update Submitted: 2021-07-05
• Last Update Posted: 2021-07-08

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: All
• Target Recruitment: Not specified

Inclusion Criteria

• Children and adults, without SARS-CoV-2 infection, in whom an adenoidectomy and/or tonsillectomy is performed for their care in the hospitals of Assistance Publique-Hôpitaux de Paris participating in the study.
• Holders of parental authority of minor patients and adult patients not opposed to participation in the study.

Exclusion Criteria

• N/A

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Adenoidectomy / tonsillectomy	Storage of operating waste	Children and adults, without SARS-CoV-2 infection, in whom an adenoidectomy and/or tonsillectomy is performed for their care in the centers of the study.

Primary Outcome Measures

Name	Time	Method
Expression of ACE 2 receptor and TMPRSS2 serine protease	9 months	Measurement of expression of ACE 2 receptor and TMPRSS2 serine protease in tonsils and adenoids labeled by immunofluorescence.

Secondary Outcome Measures

Name	Time	Method
Location of ACE 2 receptor and TMPRSS2 serine protease	9 months	Location of ACE 2 receptor and TMPRSS2 serine protease in tonsils and adenoids labeled by immunofluorescence.

Trial Locations

Locations (9)

Hôpital Mondor; 🇫🇷 Créteil, France

Hôpital Bicêtre; 🇫🇷 Le Kremlin-Bicêtre, France

Hôpital Lariboisière; 🇫🇷 Paris, France

Hôpital Armand Trousseau; 🇫🇷 Paris, France

Hôpital Pitié Salpêtrière; 🇫🇷 Paris, France

HEGP; 🇫🇷 Paris, France

Hôpital Bichat; 🇫🇷 Paris, France

Hôpital Tenon; 🇫🇷 Paris, France

Hôpital Necker-Enfants Malades; 🇫🇷 Paris, France