Role of Antimicrobial Peptides in Host Defense Against Vaccinia Virus
- Conditions
- Atopic Dermatitis
- Registration Number
- NCT00407069
- Brief Summary
Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by recurrent viral skin infections. Recent studies have demonstrated that the skin of people with AD my have decreased antimicrobial peptide (AMP) expression. The purpose of this study is to compare smallpox virus replication and the number of AMPs and other antiviral molecules in people with AD, as compared to those seen in people with psoriasis or asthma, or healthy individuals.
- Detailed Description
AD is a chronic inflammatory skin disease characterized by frequent viral skin infections. Recent studies have found that components in the skin of people with AD may block AMP expression. AMPs are responsible for preventing infection from viruses. The purpose of this study is to examine smallpox virus replication and AMP expression in the skin of patients with AD as well as identify other antiviral molecules involved in immune response. These findings will be compared with those of people with psoriasis or asthma, or healthy individuals. This study will consist of one study visit at which skin and blood samples will be taken.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 286
Not provided
- Oral corticosteroids or any systemic immunosuppressive or immunomodulatory medication within 28 days prior to study entry
- Immunotherapy within 3 months prior to study entry
- History of bleeding disorder
- Aspirin, oral antihistamines, oral antibiotics, oral cyclosporine, or topical medications within 7 days of screening visit including, but not restricted to, Protopic, Elidel, topical corticosteroids, and topical antibiotics
- Anxiolytic agents and antidepressants within 2 days of screening visit
- Diabetic requiring medication
- Autoimmune or immunodeficiency
- Active fungal, bacterial, or viral infections within 7 days prior to study entry
- Active systemic cancer. Participants with uncomplicated nonmelanoma skin cancer are not excluded.
- Theophylline or leukotriene antagonists within 24 hours of screening visit
- Received any vaccination within 30 days prior to study entry
- Known lidocaine allergy
- Previously vaccinated for smallpox
- Pregnant or breastfeeding
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Expression of vaccinia virus mRNA in non-lesional skin following inoculation with untreated vaccinia virus will be evaluated using real-time RT-PCR (Reverse transcription polymerase chain reaction). 5 years
- Secondary Outcome Measures
Name Time Method Ability of structural analogues of CSAs (Cyclosporine) to kill purified vaccinia virus as well as keratinocytes infected with vaccinia virus in vitro. 5 years Expression of cytokines, AMPs, other antiviral molecules, or epidermal differentiation proteins in non-lesional skin prior to and after inoculation with vaccinia virus will be evaluated using real-time RT-PCR. 5 years Expression of over 20,000 genes will be evaluated by GeneChip microarrays in non-lesional skin, and PBMCs stimulated with vaccinia virus. Real-time RT-PCR of skin and PBMC will be used to confirm gene alterations found in GeneChip microarrays. 5 years Keratinocytes will be stimulated with vaccinia virus in the presence and absence of Th1 or Th2 cytokines. Non-lesional AD skin will be stimulated with vaccinia virus in the presence of antibodies that neutralize Th2 cytokines. 5 years Vaccinia virus replication will be evaluated using a standard viral plaque assay in BS-C-1 cells and by analyzing vaccinia virus mRNA expression using real-time RT-PCR in keratinocytes and BS-C-1 cells. 5 years
Trial Locations
- Locations (1)
National Jewish Health
🇺🇸Denver, Colorado, United States