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Immunity and Safety of Inactivated Enterovirus 71 Vaccine Post-marketing Among Children Aged 6-35 Months in China

Phase 4
Completed
Conditions
Hand, Foot and Mouth Disease
Interventions
Biological: The first batch of inactivated EV-A71 vaccine (vero cell) producted by 40 L reactor
Biological: The second batch of inactivated EV-A71 vaccine (vero cell) producted by 40 L reactor
Biological: The first batch of inactivated EV-A71 vaccine (vero cell) producted by 150 L reactor
Biological: The second batch of inactivated EV-A71 vaccine (vero cell) producted by 150 L reactor
Biological: The third batch of inactivated EV-A71 vaccine (vero cell) producted by 40 L reactor
Biological: The third batch of inactivated EV-A71 vaccine (vero cell) proudected by 150 L reactor
Registration Number
NCT03893747
Lead Sponsor
Jiangsu Province Centers for Disease Control and Prevention
Brief Summary

This study evaluates three batches consistency, immunity duration and safety of inactivated Enterovirus 71(EV-A71) vaccine(vero cell) post-marketing among children aged 6-35months in China.3000 subjects will be recruited in this study, of who 1500 subjects will be randomly assigned in a ratio of 1:1:1 to receive one of the three batches of EV-A71 vaccines manufactured by 40L capacity reactor, other 1500 subjects will be randomly assigned in a ratio of 1:1:1 to receive one of the three batches of EV-A71 vaccines manufactured by 150L capacity reactor. Vaccine wil be administrated according to a two doses of schedule given at day 0 and 28. Sample will be collected at day 0, day 56 and at month 13 after vaccinaiton.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
3000
Inclusion Criteria
  • Healthy children aged 6-35 months
  • Subjects who have been clinically judged to be healthy by the researcher after being asked about the medical history and related physical examination
  • The subjects' guardians agree the requirements of the protocol and the relevant follow up visits
  • The subjects' guardians agree and sign the informed consent
  • Subjects who have no relocation or long-term travel plan within one year and are able to comply with the requirements of the clinical trial protocol.
Exclusion Criteria
  • Subject who has a medical history of HFMD caused by EV71 or has been vaccinated with EV71 vaccine
  • Subject that has a history of allergies to vaccines or vaccine ingredients, and has serious side effects on vaccines, such as allergies, urticaria, dyspnea, vascular neuroedema or abdominal pain
  • Subject who has congenital malformations or developmental disorders, genetic defects, severe malnutrition, etc.
  • Subject who has a history of epilepsy, convulsion or convulsion, or a family history of mental illness
  • Subject who has autoimmune disease or immune deficiency, or whose parents and siblings have autoimmune disease or immune deficiency
  • Subject who has a history of asthma, vascular and neuroedema, diabetes or malignant tumor
  • Subject who has a thyroid resection history, or received treatment due to thyroid disease in the past 12 months
  • Subject who has an abnormal coagulation function (such as coagulation factor deficiency, coagulant disease, platelet abnormality) or obvious bruising or clotting disorder which was diagnosed by doctors.
  • Asplenia, functional asplenia, or splenectomy due to any circumstances
  • Acute onset of various acute or chronic diseases in the last 7 days
  • Immunosuppressant therapy, antiallergic therapy, cytotoxicity therapy, inhaled corticosteroids (excluding corticosteroid spray therapy for allergic rhinitis, surface corticosteroid therapy for acute non-dermatitis) in the past 6 months
  • Any prior administration of blood products in last 3 months
  • Any prior administration of other research medicines or vaccines in last 1 month
  • Any prior administration of attenuated live vaccine in last 15 days
  • Any prior administration of subunit or inactivated vaccines in last 7 days
  • Under the anti-TB prevention or therapy
  • Underarm temperature > 37.0 ℃ for fever before vaccination
  • Other factors that are not suitable for clinical trials according to the judgment of researchers

Exclusion Criteria for the second dose:

  • Have severe allergic reaction after first dose
  • Have severe adverse reactions related to first dose
  • Any situation meet the exclusion criteria stated in the exclusion criteria for first dose
  • Acute infection or illness
  • Other factors that are not suitable for clinical trials according to the judgment of researchers

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
the first batch vaccine producted by 40 L reactorThe first batch of inactivated EV-A71 vaccine (vero cell) producted by 40 L reactor500 subjects will be randomly received the first batch vaccine producted by 40 L reactor
the second batch vaccine producted by 40 L reactorThe second batch of inactivated EV-A71 vaccine (vero cell) producted by 40 L reactor500 subjects will be randomly received the second batch vaccine producted by 40 L reactor
the first batch vaccine producted by 150 L reactorThe first batch of inactivated EV-A71 vaccine (vero cell) producted by 150 L reactor500 subjects will be randomly received the first batch vaccine producted by 150 L reactor
the second batch vaccine producted by 150 L reactorThe second batch of inactivated EV-A71 vaccine (vero cell) producted by 150 L reactor500 subjects will be randomly received the second batch vaccine producted by 150 L reactor
the third batch vaccine producted by 40 L reactorThe third batch of inactivated EV-A71 vaccine (vero cell) producted by 40 L reactor500 subjects will be randomly received the third batch vaccine producted by 40 L reactor
the third batch vaccine proudected by 150 L reactorThe third batch of inactivated EV-A71 vaccine (vero cell) proudected by 150 L reactor500 subjects will be randomly received the third batch vaccine producted by 150 L reactor
Primary Outcome Measures
NameTimeMethod
Incidence and severity of adverse reactions/adverse events after each dose1 month after each dose

Incidence and severity of adverse reactions/adverse events within 1 month after each dose

Consistency of different batches for geometric mean titre(GMT) after vaccination56 days after vaccination

Consistency of different batches for GMT of EV-A71 neutralizing antibody on 56 day after vaccination

Secondary Outcome Measures
NameTimeMethod
Positive rate of EV-A71 neutralizing antibody on day 56 after vaccination56 days after vaccination

Positive rate of EV-A71 neutralizing antibody on day 56 after vaccination for different batchs

GMTof EV-A71 neutralizing antibody on month 13 after vaccination13 months after vaccination

GMTof EV-A71 neutralizing antibody on month 13 after vaccination for different batchs

GMTof EV-A71 neutralizing antibody on day 56 after vaccination56 days after vaccination

GMTof EV-A71 neutralizing antibody on day 56 after vaccination for different batchs

Geometric Mean Fold Increase(GMFI) of EV-A71 neutralizing antibody on day 56 after vaccination56 days after vaccination

Geometric Mean Fold Increase(GMFI) of EV-A71 neutralizing antibody on day 56 after vaccination for different batchs

Positive rate of EV-A71 neutralizing antibody on month 13 after vaccination13 months after vaccination

Positive rate of EV-A71 neutralizing antibody on month 13 after vaccination for different batchs

Incidence of sever adverse events after vaccination13 months after vaccination

Incidence of sever adverse events within 13 months after vaccination

Seroconversion rate of EV-A71 neutralizing antibody on day 56 after vaccination56 days after vaccination

Seroconversion rate of EV-A71 neutralizing antibody on day 56 after vaccination for different batchs

Trial Locations

Locations (1)

Fengcai Zhu

🇨🇳

Nanjing, China

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