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HIIT + RT on Body Composition and Gut Microbiota in Postmenopausal Women (PACWOMan)

Not Applicable
Completed
Conditions
Overweight
Postmenopausal Women
Interventions
Other: Training program
Registration Number
NCT03940924
Lead Sponsor
Laboratoire des Adaptations Métaboliques à l'Exercice en conditions Physiologiques et Pathologiques
Brief Summary

Postmenopausal women, as men, are more prone to central or android obesity than premenopausal women. Recently, some studies have shown that obesity is associated with gut microbiota dysbiosis and gut microbiota could be responsible of fat mass accumulation. Moreover, abdominal fat mass accumulation is associated with an increase of cardiovascular disease risks.

Recent studies suggest that physical activity may positively alter gut microbiota composition. Accumulating evidence suggests that high intensity interval training (HIIT) is an effective strategy for reducing body fat of overweight individuals, especially at the abdominal level. Resistance Training (RT) is associated with increased muscle mass and strength gain in main muscle groups. Thus, RT is also an interesting modality to fight against deconditioning and autonomy loss with age.

The aim of the study was to observed the effects of a 12-week high intensity interval training (HIIT) combined with resistance training (RT) program on gut microbiota and body composition changes in postmenopausal women.

It is hypothesized that HIIT +RT will improve gut microbiota and body composition (including whole body and (intra)-abdominal fat mass) in a concomitant and parallel way.

Detailed Description

The aim of the study was to observed the effects of a 12-week high intensity interval training (HIIT) combined with resistance training (RT) program on gut microbiota and body composition changes in postmenopausal women.

30 postmenopausal women will be randomly assigned to HIIT + RT (n= 15) group or Control group (n=15).

HIIT+RT: Each subject performed HIIT protocol consisted of repeated 60 cycles of speeding up for 8 s followed by pedalling slowly for 12 s (20min) Then they performed a single set of 10 exercises with 1 or 2min resting period between exercises. The set consisted of 8-12 repetitions at about 80% maximum repetition (1RM).

Control group : (no training program) Subjects will keep their life style.

Faecal microbiota and body composition will be measured before and after the intervention (3 months).

The investigators will examine the effects of HIIT + RT programs on:

Body composition : total-abdominal and visceral fat mass, total fat free mass and muscle mass Faecal concentration of short chain fatty acids Total fat free mass (and appendicular fat free mass) (DEXA) Glycemic profile (plasma HbA1c, plasma glucose, plasma insulin) Lipid profile (TG, HDL, LDL, total cholesterol) Aerobic capacity (VO2 max) Muscular capacity (fatigability, isometric and dynamic strenght)

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
17
Inclusion Criteria
  • Postmenopausal women
  • BMI ≥ 25 and < 40
  • Able to follow an exercise protocol
  • Eating behavior and physical activity stable since at least 3 month
Exclusion Criteria
  • Subject not able to perform exercise after medical examination
  • Subject not able to perform bicycle exercise (pains)
  • Use of β-blocker
  • Medical treatment that could interfere with the different outcome measures (antibiotics)
  • Hormonal Replacement Therapy (HRT)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
High Intensity Interval Training (HIIT) + Resistance TrainingTraining programSubjects perform three sessions of training during 12 weeks. Session are composed of 20min HIIT program : 60 cycles of speeding up for 8s and pedaling slowly for 12s. (Intensity between 85 and 90% HRmax) + a single set circuits including 10 exercises with a load of 8-12 repetition at around 80% of maximal repetition (1RM)
Primary Outcome Measures
NameTimeMethod
Changes in the composition of gut microbiotaBaseline T0 and 3 months after intervention starting

Evolution of microbiota composition will be evaluated from fecal microbiota analyse before and after intervention.

Secondary Outcome Measures
NameTimeMethod
Total fat massBaseline T0 and 3 months after intervention starting

Change from baseline in total fat mass (g) (estimated from DEXA)

Total fat free massBaseline T0 and 3 months after intervention starting

Change from baseline in total fat free mass (g) (estimated from DEXA)

Evolution of lipid profileBaseline T0 and 3 months after intervention starting

Change from baseline in plasma triglycerides, total cholesterol, LDL- and HDL cholesterol.

Evolution of plasma inflammatory markerBaseline T0 and 3 months after intervention starting

Change from baseline in plasma C-Reactive Protein

Evolution of fitness levelBaseline T0 and 3 months after intervention starting

Change from baseline in VO2max

Abdominal fat massBaseline T0 and 3 months after intervention starting

Change from baseline in abdominal fat mass (g) (estimated from DEXA)

Visceral fat massBaseline T0 and 3 months after intervention starting

Change from baseline in visceral fat mass (g) (estimated from DEXA)

Concentration of Short Chain Fatty Acids (SCFAs)Baseline T0 and 3 months after intervention starting

Short Chain Fatty Acid's concentration in faeces will be measured

Evolution of fasting glucose levelBaseline T0 and 3 months after intervention starting

Change from baseline in plasma fasting glucose level

Evolution of insulinemiaBaseline T0 and 3 months after intervention starting

Change from baseline in plasma fasting insulinemia (mmol/L)

Evolution of HbA1cBaseline T0 and 3 months after intervention starting

Change from baseline in plasma HbA1c (%)

Evolution of muscular capacityBaseline T0 and 3 months after intervention starting

Change from baseline maximal dynamic strenght

Trial Locations

Locations (1)

CREPS Auvergne Rhône-Alpes / Vichy

🇫🇷

Bellerive-sur-Allier, Allier, France

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