Influence of High Salt Diet on Microvascular Reactivity in Young Healthy Subjects
Trial Snapshot
- Phase
- Not Applicable
- Enrollment
- 50
- Locations
- 1
- Primary Endpoint
- microvascular reactivity
Study Overview
Brief Summary
It is well accepted that high-salt (HS) intake is an essential risk factor in development and progression of hypertension. Results of some recent studies suggest that some of the deleterious effects of a HS diet are independent of elevated blood pressure (BP) and may occur in normotensive individuals and are associated with impaired endothelial function. However, the effects of acute salt loading on endothelial function and vascular reactivity in young healthy individuals are still scarce and inconsistent.
The purpose of present study is to determine whether one week of HS intake affects microvascular reactivity in young healthy subjects without changes in BP. In addition, the investigators sought to evaluate if potential HS diet-induced microvascular dysfunction is associated with changes in oxidative stress level and/or with modification of immunological response in young healthy subjects.
Study Design
- Study Type
- Interventional
- Allocation
- Non Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 25 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •healthy volunteers
Exclusion Criteria
- •oral contraceptives
- •drugs that could affect the endothelium
- •hypertension
- •coronary artery disease
- •hyperlipidaemia
- •renal impairment
- •cerebrovascular and peripheral artery disease
Arms & Interventions
low salt diet
All subjects will be instructed to maintain a low-sodium (LS) diet, with an intake of less than 2.3 g of salt per day (DASH eating plan; US Department of Health and Human Services, 2006) for 7 days (washout period).
Intervention: Low Salt (LS) diet (Other)
high salt diet
After washout period, all subjects will be instructed to maintain a high-sodium (HS) diet, with an intake of 11.2 g of salt per day for 7 days.
Intervention: High Salt (HS) diet (Other)
Outcomes
Primary Outcomes
microvascular reactivity
Time Frame: two weeks after starting the protocol
Cutaneous microvascular blood flow will be measured by Laser Doppler Flowmetry in response to vascular occlusion (post occlusive reactive hyperemia- PORH) and in response to iontophoresis of acetylcholine (ACh) (endothelium dependent vasodilation) before and after diet protocols.
Secondary Outcomes
- antioxidant capacity(two weeks after starting the protocol)
- oxidative stress(two weeks after starting the protocol)
- modification of immunological response by high salt diet(two weeks after starting the protocol)
Investigators
Ines Drenjancevic
Vice Dean for Science, Faculty of Medicine Josip Juraj Strossmayer University of Osijek
Josip Juraj Strossmayer University of Osijek