Comparison of Oxytocin Receptor Immunoreactivity in Placentas Obtained From Women Having Cesarean and Natural Birth
- Conditions
- Healthy Mothers Who Have Had Both Vaginal and Cesarean Deliveries
- Interventions
- Other: The delivery being performed via cesarean section surgery rather than natural childbirth
- Registration Number
- NCT06275438
- Lead Sponsor
- Kafkas University
- Brief Summary
Our study examines the effects of oxytocin hormone receptors. mechanisms of placental tissue cells and therefore intrauterine development. It was conducted to investigate the effects on newborn babies apgar score, fontanel openings, birth weights, etc. By collecting data, normal and They were compared between cesarean births by statistical analysis. normal in this regard Based on the hypothesis that there is a difference between labor and cesarean delivery, this study has been made.OXTR primary antibody was used for immunohistochemical examination. There was no significant difference between the groups in terms of histological examination. In the study, it was found that OXTR immunoreactivity was strong in decidual cells and stromal cells in villi. A stronger OXTR immunoreactivity was detected in villous stroma of placenta of the natural birth group compared to the cesarean birth group. Reaction was not observed in syncytotrophoblast cells and syncytial nodes in both groups, but strong immunoreaction was observed fetal endothelial cells and fetal capillaries in both groups.
- Detailed Description
The aim of this study was to compare oxytocin receptor (OXTR) immunoreactivity in placentas obtained from women having cesarean and natural birth. The study samples consisted of placentas of 32 mothers who had 16 natural birth (control) and 16 cesarean (case) birth in Kars Harakani State Hospital and Kafkas University Health Research and Application Hospital. At the beginning of the study, 40 placentas obtained in total, 5 were not included in the study due to mismatched blood, 2 were not included in the study after the chronic disease (thyroid, Behçet's disease) detected, and 1 was not included in the study due to the presence of accessory placenta. The means of binary variables were determined by applying the Independent Two-Sample T-Test. Pearson Correlation analysis was used to examine the relationship between the variables. Periodic acid-Schiff staining, H\&E and Triple staining techniques were applied to tissue samples obtained from placentas for histological examination. OXTR primary antibody was used for immunohistochemical examination. There was no significant difference between the groups in terms of histological examination. In the study, it was found that OXTR immunoreactivity was strong in decidual cells and stromal cells in villi. A stronger OXTR immunoreactivity was detected in villous stroma of placenta of the natural birth group compared to the cesarean birth group. Reaction was not observed in syncytotrophoblast cells and syncytial nodes in both groups, but strong immunoreaction was observed fetal endothelial cells and fetal capillaries in both groups. As a result, it is thought that the placenta pathology should be made routine for every birth because it gives information about the offspring.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 32
Mothers with a body mass index within this range:18kg/m2≥Body Mass Index (BMI)≤35kg/m2 Healthy mothers who have had both vaginal and cesarean deliveries Mothers aged over 18 and under 35 years old
- Chronic diseases Gynecological disorders Intrauterine growth restriction Placental anomalies Forensic cases of pregnancy Premature births Eclampsia-Preeclampsia Multiple pregnancies Body Mass Index (BMI) ≥35kg/m2 Body Mass Index (BMI) ≤18kg/m2 Experience of trauma during pregnancy Non-cesarean surgical interventions during pregnancy Severe infections
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description experimental group The delivery being performed via cesarean section surgery rather than natural childbirth The group consists of mothers who meet the inclusion criteria specified in the study, have an 8cm dilation at term, and undergo cesarean section delivery.
- Primary Outcome Measures
Name Time Method Oksitosin Receptor Immunoreactivity 2 weeks After subjecting the placenta samples to various biochemical tests and staining procedures, they were prepared appropriately and evaluated for oxytocin receptor activity after being incubated for 24 hours.
- Secondary Outcome Measures
Name Time Method Pearson correlation analysis 1 months The collected data included variables such as maternal age, body mass index (BMI), number of abortions, number of abortions, age at first pregnancy, number of live children, date of last menstrual period, and various newborn parameters such as gender, weight, head circumference, length, 1st and 5th minute Apgar scores, anterior-posterior fontanelle opening, placental thickness, weight, major-minor diameter, and total cord length.
For the statistical analysis of the collected data, version 20.0 of the SPSS program was utilized. Independent Samples T-Test was applied to compare the means of binary variables and their differences at a significance level of 5%. Pearson correlation analysis was employed to examine the relationships between variables.
Trial Locations
- Locations (1)
Kafkas Universty
🇹🇷Kars, Merkez, Turkey