Endocan, VEGF and TNF-alpha Levels in Periodontal Disease and After Treatment

Completed

• Conditions: Periodontal Disease
• Interventions: Procedure: Non-surgical periodontal treatment

• Registration Number: NCT02750956
• Lead Sponsor: Bulent Ecevit University

Brief Summary

The primer aim of the study was to determine serum and gingival crevicular fluid (GCF) endocan levels in periodontal diseases pathogenesis supported with vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) levels.

Detailed Description

The study consist of three groups: periodontally healthy individuals (group 1; n = 20), individuals with generalized chronic periodontitis (group 2; n = 20), the same patients with chronic periodontitis after they had been treated with scaling and root planing (SRP) were considered as Group 3. Clinical measurements were recorded; GCF and serum samples were obtained from each participant before and 6 weeks after therapy. Endocan, VEGF-A and TNF-α levels were measured by enzyme-linked immunosorbent assay.

Study Timeline

• Study Start: 2015-06
• Primary Completion: 2015-12
• Study Completion: 2016-03
• Status Verified: 2016-04
• Study First Submitted: 2016-04-19
• Study First Submitted QC: 2016-04-21
• Last Update Submitted: 2016-04-21
• Study First Posted: 2016-04-26
• Last Update Posted: 2016-04-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

• No bone and attachment loss,
• GI=0, PPD≤3mm, CAL≤3mm
• Systematically healthy patients

Group 2 Inclusion Criteria:

• GI>1, PPD≥5mm, CAL≥5mm with alveolar bone loss radiographically.
• Systematically healthy patients

Exclusion Criteria

• Aggressive Periodontitis,
• Oral pathologies,
• Patients with any other systemic diseases,
• Pregnant women and those in the lactation period,
• Patients with smoking habit and taking medication
• Patients received periodontal therapy in last 6 months

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Group 3	Non-surgical periodontal treatment	the same patients in group 2 after they had been treated with scaling and root planing (SRP) were considered as Group 3.
Group 2	Non-surgical periodontal treatment	Patients with chronic periodontitis

Primary Outcome Measures

Name	Time	Method
Endocan levels	6 weeks	Change after periodontal treatment

Secondary Outcome Measures

Name	Time	Method
TNF-alpha levels	6 weeks	Change after periodontal treatment
VEGF levels	6 weeks	Change after periodontal treatment