Q-koorts post-vaccination study.
Recruiting
- Conditions
- post-vaccinationhumoral immune responsecellular immune response.Q-fever infection
- Registration Number
- NL-OMON28463
- Lead Sponsor
- Rijks Instituut voor Volksgezondheid en Milieu (RIVM)
- Brief Summary
/A
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 280
Inclusion Criteria
1. Has taken part in the national Q-fever vaccination program;
2. Willing to adhere to blood draw schedule;
Exclusion Criteria
None.
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Humoral immune response: IgG and IgM antibodies against fase 1 and 2 antigens of C. burnetii are measured by means of several serological tests:<br /><br>1. Immune fluorescence assay (IFA);<br /><br>2. Enzyme-linked immuno sorbent assay (ELISA);<br /><br>3. Complement binding reaction (CBR);<br /><br>4. Polymerase chain reaction (PCR);<br /><br>5. Micro-array.<br><br /><br /><br>Cellular immune response: After stimulation of whole blood, the levels of interferon-gamma (IFN-gamma), interleukine (IL)-10 and possibly IL-12 production are measured. Also a T-helper 1 and T-helper 2 cytokine profile are measured in isolated mononuclear cells, isolated CD14+ monocytes and isolated T-cells after stimulation, and differentiation of the cells is studied.
- Secondary Outcome Measures
Name Time Method