A dual stable isotope method to assess dietary protein quality in humans: Characterization of intrinsically labelled 15N milk protein as a reference protei

Completed

• Conditions: geen, methodologie; methodology; non

• Registration Number: NL-OMON43541
• Lead Sponsor: Wageningen Universiteit

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 23 April 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 10

Inclusion Criteria

* Healthy
* Age: 20 * 35 y
* BMI: 18.5 * 25 kg/m2
* Stable dietary habits
* Veins suitable for cannulation (blood sampling)

Exclusion Criteria

* Having a history of medical or surgical events that may significantly affect the study outcome
* Medical drug use accept incidental use of paracetamol
* (Chronic) disease which might influence the study outcomes e.g. diabetes mellitus or any other endocrine disorder, active cardiovascular disease, hepatic disease, renal disease, cancer , bowel disease
* Milk protein or lactose intolerance or allergy
* Alcohol consumption of >14 (women) or > 21 (men) units per week
* Drug abuse
* Moderate intense physical activity (exercise) for more than 5 hours/week
* Reported weight loss or weight gain of > 3 kg in the month prior to pre-study screening
* Reported slimming diet, or medically prescribed diet
* Having a habitual diet with a protein content of <10 En% > 30 En%
* Reported vegan or macrobiotic life-style

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The ratio of isotopic enrichment (15N/13C) of (total) amino acids of the test<br /><br>meal and the blood plasma as determined by (gas chromatography*) isotope ratio<br /><br>mass spectrometry ((GC)-IRMS).</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Measurement of total N, urea and ammonia by kinetic UV assay and 15N and 13C<br /><br>enrichment by GC/IR-MS in urine will be assessed. </p><br>