Immunological and metabolic effects of dietary methionine reduction in healthy volunteers (MEDIATE)
- Conditions
- Prevention, autophagy, fasting simulation by dietary methionine restriction
- Registration Number
- DRKS00032816
- Lead Sponsor
- Charité Universitätsmedizin Berlin
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 24
Men and women in a ratio of 1:1
- Body Mass Index 18.5 - 29.9 kg/m2
- Ability to provide informed consent
- Existing health insurance to cover any necessary clarification and treatment of incidental findings.
- Diseases or functional disorders which, in the opinion of the
study doctor preclude participation in the study
- Severe, manifest and treatment-requiring diseases
- Permanent use of prescription drugs (except hormonal
contraception)
- Vitamin B6, B12 and folic acid levels below the normal range
- Homocysteinemia
- Postoperative phases
- Acute and chronic infections
- Special diet for medical reasons
- Taking food supplements
- Food intolerances
- Vegetarian or vegan diet
- Pregnancy and breastfeeding
- Smoker
- Following a diet or weight reduction programme
- Change in body weight of more than 2 kg in the
previous month of the study
- Habitual methionine intake < 20 mg/kg bw/d
- Known medication, drug or alcohol abuse
- Incapacity or other circumstances that do not allow the
subject to fully understand the nature, meaning and
implications of this study
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Detection of autophagy activation by autophagic flux (LC3-II) and mTOR (p-S6 and p-4EBP1) by flow cytometry after 14 days of dietary methionine reduction (10 mg/kg bw/d)
- Secondary Outcome Measures
Name Time Method