Assessment of Nitro - Oxidative Stress in Periodontal Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- Malondialdihyide levels in serum and gingival cervicular fluid
研究概览
简要总结
Periodontitis is a chronic inflammatory disease whose etio-pathogencity is not fully understood yet. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved in physiological and pathological processes. Nitro-oxidative stress has been implicated in Periodontitis.
The aim of this study is to assess the levels of ROS and RNS in serum and gingival crevicular fluid (GCF) samples taken from periodontitis (chronic and aggressive) patients and healthy controls. Subsequently, correlating these levels with the severity of periodontal disease.
Eighty subjects will be invited to participate in this study. Patients will be allocated into four groups (20 patients each). The biochemical parameters that will be investigated are Malondialdehyde (MDA) (using TBRSA assay) as a marker of oxidative stress and (NO- level using Griess reagent) as a marker of nitrosative stress.
详细描述
Introduction:
Teeth and their supporting periodontal structures are of great importance in good oral health. Periodontitis is an inflammatory disease of the periodontium which affects the supporting tissues of the teeth. The disease is multifaceted and its etio-pathogenecity is still not fully understood and therefore the treatment of different types of periodontal disease can be very difficult. Numerous risk factors have been implicated in the disease process including risk determinants and risk indicators. Most recently, strong body of evidence has accumulated to support a role for reactive oxygen (ROS) and nitrogen species (RNS) either as trigger agents or more frequently, aggravators of the primary lesions in periodontitis.
Oxygen and nitrogen reactive species are involved in a large number of physiological and pathological processes. ROS generated by monocytes and neutrophyles during inflammation are important aggression factors in the periodontal tissues. ROS play an important role in activation of osteoclasts and in bone resorption.
Oxidative stress is a condition arising when there is a serious imbalance between the levels of free radicals in a cell and its antioxidant defenses in favor of the former. Thus, tissue damage can result when antioxidant systems are unable to counteract the free radicals actions efficiently. Inflammation when stimulated by bacterial pathogens, host cells release proinflammatory cytokines (IL-1α, IL-6, IL-8, and tumor necrosis factor-α) as part of immune response. These cytokines recruit polymorphonuclear cells (PMNs) that play a major role in the etiology of periodontal disease by producing proteolytic enzymes, such as elastase and O2 (molecular oxygen) by the oxidative burst.
In a recent study, Abou Sulaiman & Shehadeh found that serum total antioxidant capacity (TAS) was lower in non-smokers Syrian patients with chronic periodontitis compared to healthy controls. Also, they reported that periodontal treatment restored TAS levels to normal levels similar to healthy controls.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Malondialdihyide levels in serum and gingival cervicular fluid
时间窗: Within 24 hours after the collection of the serum and gingival fluid
TBARS assay (HPLC) will be used to assess the malondialdihyde levels as a marker of oxidative stress.
Nitric oxide levels in serum and gingival cervicular fluid
时间窗: Within 24 hours after the collection of the serum and gingival fluid
Griess reagent to assess nitric oxide levels as a marker of nitrosative stress.
次要结局
- Bleeding on Probing (BOP)(One time assessment once sample recruitment has completed. This outcome will be assessed within one week before treatment provision)
- Clinical Attachment Loss (CAL)(One time assessment once sample recruitment has completed. This outcome will be assessed within one week before treatment provision)
- Plaque Index (PI)(One time assessment once sample recruitment has completed. This outcome will be assessed within one week before treatment provision)
- Periodontal Pocket Depth (PPD)(One time assessment once sample recruitment has completed. This outcome will be assessed within one week before treatment provision)
- Gingival Index (GI).(One time assessment once sample recruitment has completed. This outcome will be assessed within one week before treatment provision)