Assessment of a novel strategy to attenuate muscle mass loss during 2 weeks of bed rest

Completed

Conditions: Inactivity
Skeletal Muscle Disuse
10028302

Registration Number: NL-OMON45252

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 19

Inclusion Criteria

Healthy males
Age between 18 and 35 y
BMI between 18.5 and 30 kg/m2

Exclusion Criteria

-Smoking
-Type 2 Diabetes Mellitus
-Any back/leg/knee/neck/postural complaints *
-Any history or family history of thrombosis*
-All co-morbidities interacting with mobility and muscle metabolism of the lower limbs *(e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis)
-Myocardial infarction within the last 3 years
-Use of certain anti-coagulants (use of thrombocyte aggregation inhibitors such as Ascal, *acetylsalicylic acid, aspirin and carbasalaatcalcium is permitted. Use of other thrombocyte aggregation inhibitors will be discussed with the responsible physician) *
-Performing regular resistance training (3+ times per week, carrying out progressive training) in the previous 6 months
-Hypertension (according to WHO criteria) and/or cardiovascular disease
-A history of deep vein thrombosis (DVT) in the leg
-Having donated blood in the 3 months prior to the study

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The primary outcome measure is the amount of muscle mass in the legs before and<br /><br>after 2 weeks of bed rest with the application of blood flow restriction.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Whole-body skeletal muscle mass<br /><br>Leg and whole-body lean tissue mass<br /><br>Quadriceps muscle CSA<br /><br>Muscle fibre type specific CSA, vascularization and muscle fibre type-specific<br /><br>SC content<br /><br>Myofibrillar and mitochondrial fractional synthesis rate<br /><br>Whole-body adipose tissue<br /><br>Adipose tissue gene expression<br /><br>Leg muscle and maximal grip strength<br /><br>Oral Glucose Tolerance </p><br>