Adipose tissue depot-specific differences in adipocyte function and inflammatory mediators in patients with type 2 diabetes mellitus and cardiovascular disease

Recruiting

Conditions: Adipose tissue chronic low-graded inflammation
inflammation of the adipose tissue
10012653
10003216

Registration Number: NL-OMON33838

Lead Sponsor: niversitair Medisch Centrum Sint Radboud

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: Not specified

Target Recruitment: 45

Inclusion Criteria

Control subjects (males and females): Age: 40-60 yrs, BMI: 25-30 kg/m2, plasma glucose < 6.1mM ;Type 2 diabetes mellitus (T2DM) patients (males and females): subjects diagnosed with the disease T2DM, age: 40-60 yrs, BMI: 25-30 kg/m2;Subjects suffering cardiovascular disease (males and females): subjects diagnosed with peripheral vascular disease, history of myocardial infarction, angina pectoris, age: 40-60 yrs, BMI: 25-30 kg/m2

Exclusion Criteria

Control group: presence of a chronic or acute inflammation and/or autoimmune disorder, insulin resistance;Type 2 diabetes mellitus (T2DM) patients: presence of a chronic or acute inflammation and/or another autoimmune disorder ;Subjects suffering cardiovascular disease: presence of another chronic or acute inflammation and/or autoimmune disorder, insulin resistance

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>To determine adipocyte (dys)function in both visceral and subcutaneous adipose<br /><br>tissues in the three different study groups: adipocyte differentiation<br /><br>capacity, insulin sensitivity, and cell surface in the two different adipose<br /><br>tissue depots.<br /><br>To determine the state of inflammation in both visceral and subcutaneous<br /><br>adipose tissues in the three different study groups: measure adipose tissue<br /><br>macrophage and lymphocyte infiltration, characterize the macrophage subsets in<br /><br>the visceral and subcutaneous adipose tissue. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Anthropometric measurements: BMI and WHR; the circumference of the waist as a<br /><br>measure of degree of abdominal obesity.<br /><br>Plasma levels of: insulin, glucose, free fatty acids, lipids (total<br /><br>triglyceride and total cholesterol with the VLDL, LDL and HDL sub fractions),<br /><br>adipokines (adiponectin, leptin etc.) and cytokines (IL-1, IL-6, IL-8, MCP-1,<br /><br>TNF-a etc.).<br /><br>Activation state of thrombocytes as a surrogate marker for cardiovascular risk.</p><br>