Adipokines gene expression in visceral adipose tissue in patients with esophageal adenocarcinoma
Recruiting
- Conditions
- esophageal adenocarcinoma (cancer of the esophagus) and obesity1000054610017991
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 60
Inclusion Criteria
Patients with newly diagnosed esophageal adenocarcinoma who are referred to surgery at the Erasmus Medical Center will be included in our study. Sex- and BMI-matched patients who are referred for surgery at the Erasmus Medical Center for other reasons than esophageal cancer/ Barrett*s disease/GERD will be included as controls.
Exclusion Criteria
- Patients with severe weight loss (>10% during the last 3 months before surgery)
- Patients without information on height and weight at the time of surgery
- Patients with a BMI < 20
- Patients with severe co-morbidities or diseases with a possible effect on body weight and/or fat distribution
- Patients in the control group with a history of Barrett*s esophagus/GERD
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Collected samples will be immediately frozen in liquid nitrogen and stored at<br /><br>-80°C until total RNA preparation.<br /><br>Total RNA will be extracted from adipose tissue and expression levels of the<br /><br>genes of interest are quantified with real-time-quantitative-PCR (RTq-PCR). We<br /><br>will use at least 2 housekeeping genes as an endogenous reference to compensate<br /><br>for variations in input RNA amounts.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Dependent on the results of our study on gene expression we will possibly<br /><br>measure the size of adipocytes in those 2 groups later in this study.<br /><br>Previously validated methods include: direct microscopy and manual/automated<br /><br>measurement of diameters from digital images.<br /><br><br /><br>Influence of other patient characteristics on adipokines production/development<br /><br>of cancer:<br /><br>Patient information on prior weight loss, tumor stage and chronic diseases will<br /><br>be collected from the hospital information system and preoperative status.<br /><br><br /><br>Other study parameters:<br /><br>Patient information on smoking, alcohol use, use of PPI and LES-relaxing and<br /><br>other drugs, history of reflux, DM, preoperative plasma-glucose-values as well<br /><br>as levels of HbA1c, cholesterol, triglycerides, CRP etc. will be collected from<br /><br>the patients* charts/ Elpado hospital information system if available. </p><br>