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mNGS for Detection of Pathogens for Pulmonary Infection

Conditions
Pulmonary Infection
Registration Number
NCT03798925
Lead Sponsor
Sir Run Run Shaw Hospital
Brief Summary

Pulmonary infections remain the leading causes of morbidity and mortality among patients worldwide. Pathogen identification is crucial yet difficult for the majority of the cases. Metagenomic Next-generation Sequencing provides a potential technology for rapid and untargeted pathogen detection for pulmonary infection. The study is designed observationally to investigate if mNGS is superior to traditional paradigm of serial tests in the aspect of diagnostic performance. Patients whose primary diagnosis is pulmonary infetion and bronchoalveolar lavage fluid can be obtained will be enrolled. Both mNGS and traditional paradigm of serial tests wil be performed.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
100
Inclusion Criteria
  • Pulmonary infection as the primary diagnosis
  • Bronchoalveolar lavage fluid can be obtained
Exclusion Criteria
  • when the primary diagnosis is tumor or connective tissue diseases
  • microbiological diagnosis is confirmed before bronchoalveolar lavage
  • not enough bronchoalveolar lavage fluid for mNGS

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
microbiological diagnosis (pathogen of pulmonary infection)through study completion, up to 6 months

the final microbiological diagnosis (pathogen of pulmonary infection) after using of every detection method and combined with clinical manifestations.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Sir Run Run Shaw Hospital

🇨🇳

Hangzhou, Zhejiang, China

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