Mobilisation of neutrophils and premature photoaging in chronic vitiligenous skin of black skinned patients

Completed

Conditions: 1) elastosis solaris
sun damaged skin 2) vitiligo
white spot disease
10014982

Registration Number: NL-OMON31353

Lead Sponsor: niversitair Medisch Centrum Utrecht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 12

Inclusion Criteria

Group I and II: adult, male or female, aged 18 years or over; patients with skin phototype VI and vitiligo; the patient must be willing and able to give written informed consent.
Group I specifically: chronic vitiligo, persistant for 10 years or more, localised on sunexposed skin.
Group II specifically: it must be technically possible to determine the minimal erythemal dose.

Exclusion Criteria

sunlight allergy; tendency to hypertrophic scar or keloid formation; history of alcohol or drug abuse; treatment with phototherapy or systemic (immunosuppressive) therapy such as oral steroids and cyclosporin A during the study, or within 24 weeks prior to the study; treatment with oral and local antibiotics; treatment with topical steroids or tar in the tested locations during the last 2 weeks; clinically relevant cardiovascular, gastrointestinal, liver or renal disease and/or unstable metabolic or endocrine disorders; acute or chronic local bacterial, viral or fungal diseases; women of childbearing potential not using reliable contraception; pregnancy or breast feeding; psychiatric disease or history of noncompliance which, in the investigator*s assessment would interfere with appropriate protocol treatment and monitoring.

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>(a) Qualitative comparison of extra cellular matrix damage in longstanding sun<br /><br>exposed vitiligenous skin and adjacent normal skin using standard haematoxylin<br /><br>eosin and Elastica von Gieson staining procedures.<br /><br>(b) Quantitative comparison of infiltrating neutrophils in vitiligenous and<br /><br>adjacent normal skin following exposure to an equal physical dose of SSR.<br /><br>(c) Determining the distribution of DNA photoproducts in vitiligenous and<br /><br>adjacent normal skin following exposure to SSR.<br /><br>(d) Determining the MED of Vitiligenous skin. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>(a) Quantitative comparison of photoaging associated proteolytic and/or<br /><br>neutrophil associated enzymes and cytokines: MMP-1, MMP-3, MMP-8, MMP-9,<br /><br>Neutrophil elastase and IL-10 in irradiated vitiligenous and adjacent normal<br /><br>skin.<br /><br>(b) Measurement of surface markers of activation on infiltrating neutrophils:<br /><br>CD11b, CD66b, CD63.<br /><br>(c) Comparison of keratinocyte activation and keratinocyte apoptosis in<br /><br>vitiligenous and adjacent normal skin following exposure to SSR.</p><br>