Total, Free, and Bioavailable 25-Hydroxyvitamin D Levels in Patients With Periodontitis (Stage III): A Case-Control Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 83
- 试验地点
- 1
- 主要终点
- Calculation of Bioavailable 25(OH)D
研究概览
简要总结
Periodontitis; is an inflammatory disease of tissues characterized by bone destruction around the teeth. Vitamin D plays an important role in several inflammatory diseases by promoting the expression of inflammatory cytokines such as interleukin IL-1, IL-6, and IL-1β, which are directly related to periodontal attachment loss and bone demineralization. These facts suggest that vitamin D may be associated with periodontal disease. Many studies have been conducted on the relationships between serum 25-hydroxyvitamin D (25(OH)D) levels, periodontal disease, and tooth loss. However, as far as we know, there is no study on the relationship between periodontitis and free and bioavailable 25 (OH) vitamin D. In our study, over the age of 35; two groups will be formed, consisting of 40 healthy and 40 periodontitis individuals. Serum total 25 (OH) D level and vitamin D binding protein level will be analyzed by ELISA method, and bioavailable 25 (OH) D level will be calculated using total 25 (OH) D and vitamin D binding protein levels. The aim of this study; To evaluate the total, free and bioavailable 25 (OH) vitamin D levels of patients with periodontitis by comparing them with healthy controls, and to investigate whether bioavailable 25 (OH) D plays a role in the pathogenesis of periodontitis or can be a simple marker of disease activity in the light of these findings.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Other
入排标准
- 年龄范围
- 35 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Without systemic disease,
- •Had a least 14 teeth
排除标准
- •Individuals with a history of systemic or topical oral antimicrobial therapy use in the last 3 months,
- •Individuals with an allergy to any ingredient used in the study,
- •Pregnant or breastfeeding women,
- •Using vitamin D,
- •Individuals diagnosed with obesity
结局指标
主要结局
Calculation of Bioavailable 25(OH)D
时间窗: Within one week of collecting blood samples from all participants.
It will be calculated using calculations using affinity constants and calculated free 25(OH)D and measured albumin levels. Kalb = affinity constant between vitamin D and albumin = 6 x 10\^5 M\^-1 Bioavailable 25(OH)D concentration = (6 x 10\^5 x \[Albumin\]+1) x calculated free 25(OH)D Results will be recorded in ng/mL.
Measurements of 25(OH)D
时间窗: Within one week of collecting blood samples from all participants.
Total 25(OH)D levels in serum samples will be measured by ELISA method, using the "Human 25-Dihydroxy vitamin D ELISA Kit" (BT LAB, Cat No: E1981Hu, China) in accordance with the manufacturer's instructions. Results will be recorded in ng/mL.
Calculation of Bioavailable Free 25(OH)D
时间窗: Within one week of collecting blood samples from all participants.
It will be calculated using calculations using affinity constants and measured total serum 25(OH)D, VDBP and albumin levels. Kalb = affinity constant between vitamin D and albumin = 6 x 10\^5 M\^-1 KVDBP = affinity constant between vitamin D and VDBP = 7 x 10\^8 M\^-1 \[Albumin\] = serum albumin concentration = (serum albumin g/L) / 66.430 g/mol \[VDBP\] = serum vitamin D binding protein concentration = (serum VDBP g/L) / 58.000 g/mol Free 25(OH)D concentration = Total 25(OH)D / 1+(6 x 10\^5 x \[Albumin\]) + (7 x 10\^8 x \[VDBP\]) Results will be recorded in pg/mL.
Measurements of VDBP
时间窗: Within one week of collecting blood samples from all participants.
VDBP levels in serum samples will be studied by ELISA (Enzyme Linked Immunosorbend Assay) method using Elabscience brand kit (Elabscience Biotechnology Co., Ltd). Results will be recorded in (ug/mL.
Measurements of Albumin
时间窗: Within one week of collecting blood samples from all participants.
Serum albumin levels (g/dL) will be measured on the Roche Cobas c702 instrument (Germany) using commercial kits.
次要结局
- Dental Plaque(Day 1)
研究者
Tugba Aydin
Assistant Professor
Ataturk University