Endothelium-Dependent Vasoreactivity in Nitric Oxide Synthase Gene (Glu298Asp) Polymorphism: Studies in Healthy Volunteers
试验速览
- 阶段
- 1 期
- 入组人数
- 100
- 试验地点
- 2
- 主要终点
- Study I: Venous responsiveness assessed by dorsal hand vein compliance technique
研究概览
简要总结
The objective of the study is to assess the impact of genetic variation (especially polymorphisms of the gene coding endothelial nitric oxide synthase (eNOS) and the bradykinin B2 receptor gene) on venous and arterial responsiveness to vasodilators in healthy individuals without cardiovascular risk factors.
详细描述
Study I: Using the dorsal hand vein compliance technique dose-response curves to bradykinin and sodium nitroprusside will be obtained in healthy volunteers during preconstriction with phenylephrine and after pretreatment with a single dose of 500 mg i.v. acetylsalicylic acid.
Study II: Using venous occlusion plethysmography dose-response curves to acetylcholine, sodium nitroprusside and L-NG-monomethyl-arginine (L-NMMA) will be obtained in healthy volunteers pretreated with a single dose of 500mg i.v. acetylsalicylic acid.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Ect
- 盲法
- Double
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •age: 18 - 70
- •gender: male
- •good state of health
排除标准
- •known condition causing endothelial dysfunction (e.g. diabetes, hyperlipidaemia, arterial hypertension, smoking, homocysteinemia)
- •regular medication and/or treatment with drugs within the last 4-6 weeks (exclusion has to be decided in each case)
- •acute or chronic illness
- •methylxanthines and alcohol during 12 hours before the study
- •nicotine during 1 year before the study
- •drug and/or alcohol abuse
- •pregnancy or lactation
结局指标
主要结局
Study I: Venous responsiveness assessed by dorsal hand vein compliance technique
Study II: Forearm blood flow assessed by venous occlusion plethysmography
次要结局
未报告次要终点