The Obese Taste Bud Study
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 210
- 试验地点
- 1
- 主要终点
- Identification of differential gene and protein regulation in isolated human taste cells between lean and obese subjects
研究概览
简要总结
The sense of taste is essential for priming food preferences and is therefore important for caloric uptake and body weight regulation. Recent studies show that obesity is linked to changes in taste sensation but also to a loss of taste buds on the tongue. This study aims to evaluate underlying mechanisms within the taste buds to potentially influence the sense of taste on the level of these sensory cells and in order to develop new treatment strategies to fight obesity.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 69 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •normal weight (BMI 18.5-24.9)
- •obesity (BMI >=30)
- •overall healthy (no severe sickness, no cancer)
- •written informed consent
排除标准
- •current pregnancy or currently breastfeeding
- •cancer or tumors
- •state after radiotherapy at head-neck area
- •state after or current chemotherapy
- •severe psychiatric-, cardiac-, kidney- or neurological illness
- •diagnosed diabetes mellitus
- •known dysfunction of smelling or tasting
- •drug or alcohol abuse
- •permanent medication with antidepressants, anticonvulsants, steroids and/or immunosuppressants
结局指标
主要结局
Identification of differential gene and protein regulation in isolated human taste cells between lean and obese subjects
时间窗: through study completion, an average of 2 years
Transcriptomics (RNAsequencing (seq), single cell seq) and epigenomics (open chromatin mapping (ATACseq), Infinium MethylationEPIC Array) will be performed in isolated papillae fungiformes. Validation analyses by in vitro primary cell cultures of taste cell biopsy
Identification of correlations between taste cell biology with parameters of intervention
时间窗: 1 year
corrleation analyses will include factors of eating behavior, food preferences, life style factors, data from taste and smell screenings, anthropometric data, blood and saliva parameters
次要结局
- Differences of salivary and tongue microbiome on taste cells in obesity in contrast to lean(2 years)
- extracellular vesicle analyses of saliva in lean and obese subjects(2 years)
研究者
Imke Schamarek
Clinician Scientist
University of Leipzig