Inflammation and Cell Maturation in Preterm Delivery Placentas - in Vitro and in Vivo Effect of Progesterone
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 115
- 试验地点
- 1
- 主要终点
- Maturation effects of progesterone on preterm and term placentas
研究概览
简要总结
The aim of this study is to investigate the In Vitro and In Vivo effect of progesterone on immature myeloid cells (IMC), inflammation characteristics and maturation into dendritic cells (DC).
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Placentas from term and preterm deliveries
- •Singleton pregnancy
- •Consent to participate
排除标准
- •Pregnancies with obstetric complications
- •Multiple gestation
- •Maternal illness or fetal malformation known pre-delivery
研究组 & 干预措施
Preterm labor placentas and progesterone
IMC cells taken from preterm labor placentas after delivery will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with progesterone (Biological)
Term labor placentas and progesterone
IMC cells taken from term labor placentas after delivery will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with progesterone (Biological)
Preterm labor placentas without progesterone
IMC cells taken from preterm labor placentas after delivery will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture without progesterone (Biological)
Term labor placentas without progesterone
IMC cells taken from term labor placentas after delivery will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture without progesterone (Biological)
Term placentas from progesterone treated pregnancies
IMC cells taken from term labor placentas from women that were treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with growth factors (Biological)
Preterm placentas from progesterone treated pregnancies
IMC cells taken from preterm labor placentas from women that were treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with growth factors (Biological)
Term placentas not treated with progesterone in pregnancy
IMC cells taken from term placentas from women who were not treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with growth factors (Biological)
Preterm placentas not treated with progesterone in pregnancy
IMC cells taken from preterm placentas from women who were not treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with growth factors (Biological)
Term postpartum blood samples with progesterone
CD-14+ monocyte cells taken from women delivering at term will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with progesterone (Biological)
Preterm postpartum blood samples with progesterone
CD-14+ monocyte cells taken from women delivering preterm will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture with progesterone (Biological)
Term postpartum blood samples without progesterone
CD-14+ monocyte cells taken from women delivering at term will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture without progesterone (Biological)
Preterm postpartum blood samples without progesterone
CD-14+ monocyte cells taken from women delivering preterm will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells.
干预措施: Culture without progesterone (Biological)
结局指标
主要结局
Maturation effects of progesterone on preterm and term placentas
时间窗: One year
Differences observed in maturation of IMC cells into DC cells in term and preterm placentas
Inflammation characteristic effects of progesterone on preterm and term placentas
时间窗: One year
Differences observed in cytokine profiles of IMC cells from term and preterm placentas cultured with and without progesterone
次要结局
未报告次要终点