Evaluation Of Gingival Crevicular Fluid And Salivary Ferritin, Alkaline Phosphatase And Matrix Metalloproteinase-8 Levels Of Individuals With Different Periodontal Diseases
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- The total amount of ALP in GCF and saliva
研究概览
简要总结
The aim of this clinical study was to determine the levels of ferritin, alkaline phosphatase and matrix metalloproteinase-8 in the gingival crevicular fluid (GCF) and saliva samples were collected from 60 systemically healthy, non-smoker periodontitis (P group, n = 20), gingivitis (G group, n = 20) and periodontally healthy individuals (S group, n = 20) and all clinical parameters were recorded for all groups at baseline. GCF and serum samplings and clinical records were also repeated at 1st and 3rd months visitsfor only P group. Ferritin, MMP8, and ALP levels in samples were determined by enzyme-linked immunosorbent assay (ELISA).
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy
- •At least twenty permanent teeth in the mouth
- •Non-smoker
- •No medication for continuous use
- •Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- •Not in pregnancy or lactation period.
- •For the periodontitis group that has not received periodontal treatment in the last 6 months
- •For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
- •For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
- •For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
排除标准
- •Any oral or systemic disease
- •Regularly using a systemic medication
- •During pregnancy or lactation
- •Received periodontal treatment within the last 6 months.
- •Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
- •Smokers are not included in the study
结局指标
主要结局
The total amount of ALP in GCF and saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Ferritin in GCF and saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
次要结局
- The total amount of MMP-8 in GCF and saliva(24 hours after taking the clinical measurements at the first visit)
研究者
Erim Bozkurt
Research Assistant
Izmir Katip Celebi University