Sperm DNA Fragmentation Among Other Variableas a Predictor for Outcome After Varicocelectomy,
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 1
- 主要终点
- we will assess sperm DNA fragmentation as a pridector for outcome of varicoelectomy in a group of infertile males with palpable varicocele.
研究概览
简要总结
Varicocele is often associated with infertility and reduction in sperm quality, and it is one of the most common causes of reversible male infertility.
The exact mechanism of infertility caused due to varicocele formation remains unclear. One of the suggested theories is related to increased oxidative stress that causes sperm DNA damage, including DNA fragmentation, and is correlated with the decreased capacity of spermatozoa to fertilize oocytes during normal fertilization and assisted reproduction techniques. This is supported by the evidence that there is a higher prevalence of DNA fragmentation in varicocele patients and a correlation between the presence of varicocele and impaired sperm DNA integrity.
Several tests have been developed to better assess the fertility potential of sperm, including those designed to measure sperm deoxyribonucleic acid (DNA) fragmentation (SDF). The sperm DNA fragmentation index (DFI) is a potential parameter for fertility investigation, reflecting sperm DNA integrity and damage.
详细描述
Varicocele is often associated with infertility and reduction in sperm quality, and it is one of the most common causes of reversible male infertility.
The prevalence of clinically relevant varicocele ranges from 5 to 20%. It has affected 19-41% of men with primary infertility and 45-81% of men with secondary infertility.
The exact mechanism of infertility caused due to varicocele formation remains unclear. One of the suggested theories is related to increased oxidative stress that causes sperm DNA damage, including DNA fragmentation, and is correlated with the decreased capacity of spermatozoa to fertilize oocytes during normal fertilization and assisted reproduction techniques. This is supported by the evidence that there is a higher prevalence of DNA fragmentation in varicocele patients and a correlation between the presence of varicocele and impaired sperm DNA integrity.
Several tests have been developed to better assess the fertility potential of sperm, including those designed to measure sperm deoxyribonucleic acid (DNA) fragmentation (SDF). The sperm DNA fragmentation index (DFI) is a potential parameter for fertility investigation, reflecting sperm DNA integrity and damage.
Current guidelines recommend that varicoceles be treated in cases of documented infertility, palpable varicocele, normal or potentially corrected female fertility, and at least one abnormal sperm parameter with no clear statement about abnormal sperm DNA or function Furthermore, Zavattaro et al. reported that around 15% of varicocele patients with normal semen analysis are diagnosed as infertile .
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Patients have been diagnosed with VC (GII and GIII) by physical examination and scrotal color Doppler ultrasonography.
- •Patients with stable relationships.
- •In the control group, those with high SDF.
- •Patients with documented infertility.
- •Normal female partners or having correctable cause of infertility.
- •Patients age from 20 to 45 years old.
排除标准
- •Patients with a history of scrotal and spermatic cord injuries and congenital genitourinary abnormalities.
- •Patients with systemic or endocrine disease, cryptorchidism, hypogonadism, genital infection, hormonal treatment and azoospermic patients.
- •Recurrent varicocele.
- •Patients with any other disease that may cause VC (such as external kidney tumor, hydronephrosis, etc.)
- •Smoker patients and others risk factors (x ray, nuclear) workers
- •Abnormal or uncorrectable female factor. 7- Single patient.
结局指标
主要结局
we will assess sperm DNA fragmentation as a pridector for outcome of varicoelectomy in a group of infertile males with palpable varicocele.
时间窗: 1 year
In this study, we will assess sperm DNA fragmentation index as a pridector for outcome of varicoelectomy in a group of infertile males with palpable varicocele.
次要结局
未报告次要终点
研究者
Ali Deif Abdelghani Ghidan
Principal Investigator
Assiut University