Endothelial Extracellular Vesicles as Biomarkers of Blood Pressure-Induced Endothelial Activation in Pregnant Women With Hypertensive Disorders of Pregnancy
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- Plasma endothelial extracellular vesicle concentration
研究概览
简要总结
Hypertensive disorders of pregnancy are associated with endothelial dysfunction and increased maternal and fetal morbidity. Endothelial extracellular vesicles (EEVs) have emerged as promising biomarkers of endothelial activation; however, their relationship with blood pressure and endothelial function during pregnancy remains incompletely understood. This observational case-control study aims to compare circulating EEV concentrations between normotensive pregnant women and pregnant women with hypertensive disorders of pregnancy. The study will also investigate the associations between EEV levels, blood pressure, microvascular endothelial function assessed by laser Doppler flowmetry, and circulating biomarkers of endothelial activation. The findings may improve understanding of endothelial injury during pregnancy and support the development of non-invasive biomarkers for early detection and monitoring of hypertensive disorders of pregnancy.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Pregnant women aged ≥18 years.
- •Singleton pregnancy.
- •Ability to provide written informed consent.
- •Normotensive pregnancy or diagnosis of gestational hypertension or preeclampsia according to current clinical guidelines.
- •Ability to comply with study procedures.
排除标准
- •Chronic hypertension diagnosed before pregnancy.
- •Multiple pregnancy.
- •Pre-existing diabetes mellitus.
- •Chronic kidney disease.
- •Autoimmune disease.
- •Known cardiovascular disease.
- •Active infection or inflammatory disease.
- •Malignancy.
- •Inability or unwillingness to provide informed consent.
结局指标
主要结局
Plasma endothelial extracellular vesicle concentration
时间窗: Baseline
Concentration of circulating endothelial extracellular vesicles quantified by flow cytometry following size-exclusion chromatography isolation.
次要结局
- Post-occlusive reactive hyperemia (PORH)(Baseline)
- Acetylcholine-induced vasodilation (AChID)(Baseline)
- Sodium nitroprusside-induced vasodilation (SNPID)(Baseline)
- VCAM-1 concentration(Baseline)
- ICAM-1 concentration(Baseline)
- Endocan concentration(Baseline)
- von Willebrand factor (vWF)(Baseline)
- Matrix metalloproteinase-9 (MMP-9)(Baseline)
研究者
Ines Drenjancevic
Full Proffesor Tenure, Head of Department of Physiology and Immunology, Faculty of Medicine Osijek
Josip Juraj Strossmayer University of Osijek