PPAR-γ, RXR-α, and VDR Expressions in Periodontitis Patients With Different Stage and Grade Involvement
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 45
- 试验地点
- 1
- 主要终点
- Fibroblasts counts
研究概览
简要总结
Objective: The present study aimed to determine the expressions of nuclear receptors PPAR (Peroxisome proliferative activator receptor)-γ, RXR (Retinoid X receptor)-α, and vitamin D receptor (VDR) in healthy volunteers and periodontitis patients with different grade involvement.
Methods: Group-1; healthy individuals, Group-2; periodontitis patients-stage 3 grade B, (H-GradeB), Group-3; periodontitis patients-stage 3 grade C, (D-GradeC). Clinical parameters as plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were measured. Fibroblast and inflammatory cells, PPAR-γ, RXR-α, and VDR levels were determined in histological slides.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •systemic health
- •no drug use
- •no previous periodontal treatment within six months
- •no tobacco use
排除标准
- •pregnant or lactating women
- •patients used antibiotics in the last 6 months
结局指标
主要结局
Fibroblasts counts
时间窗: During histological tissue processing
For cell counting, connective tissue neighboring gingival epithelium was marked, and a cell counting frame of 10000 µm2 was marked. The fibroblast counts within the frame were counted. The measurements were performed from three different points, and the mean of these three measurements was recorded.
Vitamin D receptor (VDR)
时间窗: During histological tissue processing
Vitamin D receptor (VDR) levels were determined via immunohistochemistry.
Inflammatory cell counts
时间窗: During histological tissue processing
Connective tissues were evaluated, and inflammatory cell infiltration were counted in the H\&E stained slides using a light microscope (Nikon Eclipse, E 600, Tokyo, Japan). For cell counting, connective tissue neighboring gingival epithelium was marked, and a cell counting frame of 10000 µm2 was marked. Inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted. The measurements were performed from three different points, and the mean of these three measurements was recorded.
PPAR (Peroxisome proliferative activator receptor)-γ
时间窗: During histological tissue processing
PPAR (Peroxisome proliferative activator receptor)-γ levels were determined via immunohistochemistry.
RXR (Retinoid X receptor)-α
时间窗: During histological tissue processing
RXR (Retinoid X receptor)-α levels were determined via immunohistochemistry.
次要结局
未报告次要终点
研究者
Özkan Karataş
Assistant Professor
Tokat Gaziosmanpasa University