The Importance of Non-essential Amino Acids for Maintaining Skeletal Muscle Protein Synthesis Rates in Healthy Young Men
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 64
- 试验地点
- 1
- 主要终点
- Integrated skeletal muscle protein fractional synthesis rates
研究概览
简要总结
Background: Protein intake is important for maintaining skeletal muscle mass. These proteins consist of a collection of small building blocks which are called amino acids. There are two types of amino acids that are needed to build muscle: essential amino acids (EAA) and non-essential amino acids (NEAA). EAAs cannot be made in the body and must be consumed through foods, while NEAAs can be made within the body. However, if NEAA are not consumed through foods, the process of making NEAA in the body costs the body energy. It is still unknown what the impact of a diet containing too few non-essential amino acids is for muscle building and the body's energy metabolism.
Objective: To determine if a diet lacking NEAA, with or without being replaced by additional EAA, influences muscle protein building and whole-body metabolism.
Study design: Randomized, parallel design, double-blind, 10-day dietary intervention study.
Study population: 45 healthy (BMI 22-30 kg/m2) young males (age: 18-35 y inclusive).
Hypothesis: It is hypothesized that a diet lacking NEAA, with or without being replaced by additional EAA, decreases muscle protein building in humans.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Age between 18 and 35 y inclusive
- •BMI between 22 and 30 kg/m2
- •Non-smoker
- •Having given informed consent
排除标准
- •Participating in a structured (progressive) exercise program
- •Diagnosed GI tract disorders or diseases
- •Diagnosed musculoskeletal disorders
- •Diagnosed metabolic disorders (e.g. diabetes)
- •Cardiovascular disease
- •Hypertension (blood pressure above 140/90 mmHg)
- •Donated blood 3 months prior to test day
- •Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories).
- •Chronic use of gastric acid suppressing medication
- •Chronic use of anti-coagulants
- •Any intolerance to foods included in the standardized diet intervention
- •Any implants that would be a contra-indication for performing an MRI scan
结局指标
主要结局
Integrated skeletal muscle protein fractional synthesis rates
时间窗: 10 days
Calculated by the change in deuterium labelled alanine in skeletal muscle protein
次要结局
- Integrated skin protein fractional synthesis rates(10 days)
- Muscle mass(Baseline, 10 days)
- Body fat composition(Baseline, 10 days)
- Resting energy expenditure(Baseline, 10 days)
- Systolic and diastolic blood pressure(Baseline, 5 days, 10 days)
- Resting heart rate(Baseline, 5 days, 10 days)
- Plasma amino acids(Baseline, 5 days, 10 days)
- Blood metabolites(Baseline, 5 days, 10 days)
- Urine metabolites(Baseline, 5 days, 10 days)
- Skeletal muscle mitochondrial respiration(Baseline, 10 days)
- Skeletal muscle signalling pathways(Baseline, 10 days)
- Body mass(Baseline, 5 days, 10 days)
- Dietary protein intake(10 days)
- Dietary fat intake(10 days)
- Liver fat(Baseline, 10 days)
- Body lean mass composition(Baseline, 10 days)
- Dietary carbohydrate intake(10 days)