Effect of Non-essential Amino Acids on Protein Requirements for Endurance Athletes Using Indicator Amino Acid Oxidation Method.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 7
- 主要终点
- 13CO2 (carbon dioxide) excretion rate (μmol/kg/h)
研究概览
简要总结
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).
The biological value of protein depends on its amino acid composition. Essential amino acids are believed to have main role on whole body protein metabolism. However, it remains to be determined what role non-essential amino acids (NEAA) may have in regulating protein metabolism and contributing to the increased protein requirements after endurance exercise.
The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. However, the IAAO method has never been utilized for assessing the effect of NEAA on protein requirements after endurance exercise.
The objective of the current study is to investigate the importance of NEAA for endurance-trained male subjects.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •endurance-trained participants who regularly run more than 40 km/week
- •Participants who are categorized at least "very good" based on a study by Shvartz & Reibold, in which VO2peak is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y)
- •Ability to complete the 16-km familiarization run in session 2
排除标准
- •Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
- •Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
- •Regular tobacco use
- •Illicit drug use
研究组 & 干预措施
BASE egg protein
0.8 g/kg/d of BASE protein provided as crystalline amino acid made after egg protein.
干预措施: BASE egg protein (Dietary Supplement)
BCAA-enriched egg protein
branched-chain amino acid-enriched egg protein
干预措施: BCAA-enriched egg protein (Dietary Supplement)
small amount of essential amino acids
small amount of essential amino acids made after egg protein, which is equivalent to the amount of essential amino acids in BASE
干预措施: small amount of essential amino acids (Dietary Supplement)
large amount of essential amino acids
large amount of essential amino acids made after egg protein, which is equivalent to the amount of amino acids in BCAA
干预措施: large amount of essential amino acids (Dietary Supplement)
结局指标
主要结局
13CO2 (carbon dioxide) excretion rate (μmol/kg/h)
时间窗: at 8 hours after the end of exercise]
8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.
次要结局
- [13C]phenylalanine oxidation rate (μmol/kg/h)(at 8 hours after the end of exercise)
研究者
Daniel Moore
Assistant Professor
University of Toronto