Application of Indicator Amino Acid Oxidation Method to Investigate the Rate-limiting Amino Acids in Endurance Trained Athlete
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 5
- 主要终点
- 13CO2 (carbon dioxide) excretion rate (μmol/kg/h)
研究概览
简要总结
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).
The biological value of protein depends on the amino acid composition. As liebig's law of the minimum, the protein synthesis is limited due to the lack of single rate limiting amino acid. In this case, the first rate limiting amino acid determines protein intakes to maximize the protein synthesis. Adding a small amount of rate-limiting amino acid to the diet improves the biological value of its protein diet.
The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. however, the IAAO method has never been utilized for determining the first rate limiting amino acid.
Therefore, the purpose of this study is to address of IAAO method to be applied for determining the rate-limiting amino acid in endurance athlete.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Endurance-trained participants who regularly more than 40 km/week
- •Ability to perform the exercise stimulus (20 km run) on metabolic trial.
排除标准
- •Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
- •Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
- •Regular tobacco use
- •Illicit drug use
研究组 & 干预措施
Base protein
0.8 g/kg/d of protein provided as crystalline amino acid made after egg protein.
干预措施: Base protein (Dietary Supplement)
Sufficient protein
1.75 g/kg/d protein provided as crystalline amino acid made after egg protein.
干预措施: Sufficient protein (Dietary Supplement)
Base + BCAA
Base protein intake + Branched chain amino acids
干预措施: Base protein (Dietary Supplement)
Base + BCAA
Base protein intake + Branched chain amino acids
干预措施: Branched chain amino acids (Dietary Supplement)
Base + EAA
Base protein intake + essential amino acids.
干预措施: Base protein (Dietary Supplement)
Base + EAA
Base protein intake + essential amino acids.
干预措施: Essential amino acids (Dietary Supplement)
Base + NEAA
Base protein intake + non essential amino acids
干预措施: Base protein (Dietary Supplement)
Base + NEAA
Base protein intake + non essential amino acids
干预措施: Non essential amino acids (Dietary Supplement)
结局指标
主要结局
13CO2 (carbon dioxide) excretion rate (μmol/kg/h)
时间窗: At 8 hours after the end of exercise
8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.
次要结局
- [13C]phenylalanine oxidation rate (μmol/kg/h)(at 8 hours after the end of exercise)
研究者
Daniel Moore
Assistant professaor
University of Toronto