Application of Indicator Amino Acid Oxidation Method to Investigate the Rate-limiting Amino Acids in Endurance Trained Athlete
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Increased Metabolic Requirement
- Sponsor
- University of Toronto
- Enrollment
- 5
- Primary Endpoint
- 13CO2 (carbon dioxide) excretion rate (μmol/kg/h)
- Status
- Completed
- Last Updated
- 7 years ago
Overview
Brief Summary
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).
The biological value of protein depends on the amino acid composition. As liebig's law of the minimum, the protein synthesis is limited due to the lack of single rate limiting amino acid. In this case, the first rate limiting amino acid determines protein intakes to maximize the protein synthesis. Adding a small amount of rate-limiting amino acid to the diet improves the biological value of its protein diet.
The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. however, the IAAO method has never been utilized for determining the first rate limiting amino acid.
Therefore, the purpose of this study is to address of IAAO method to be applied for determining the rate-limiting amino acid in endurance athlete.
Investigators
Daniel Moore
Assistant professaor
University of Toronto
Eligibility Criteria
Inclusion Criteria
- •Endurance-trained participants who regularly more than 40 km/week
- •Ability to perform the exercise stimulus (20 km run) on metabolic trial.
Exclusion Criteria
- •Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
- •Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
- •Regular tobacco use
- •Illicit drug use
Outcomes
Primary Outcomes
13CO2 (carbon dioxide) excretion rate (μmol/kg/h)
Time Frame: At 8 hours after the end of exercise
8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.
Secondary Outcomes
- [13C]phenylalanine oxidation rate (μmol/kg/h)(at 8 hours after the end of exercise)