Exercise Dose-response and Protein Requirements

Not Applicable

Completed

• Conditions: Exercise Training
• Interventions: Other: Endurance exercise

• Registration Number: NCT06138951
• Lead Sponsor: University of Toronto

Brief Summary

Study will investigate the impact of training volume on protein metabolism to estimate the impact on daily protein requirements of endurance athletes.

Detailed Description

Not available

Study Timeline

• Study Start: 2017-11-17
• Primary Completion: 2018-08-15
• Study Completion: 2019-01-01
• Study First Submitted: 2023-11-09
• Study First Submitted QC: 2023-11-15
• Study First Posted: 2023-11-18
• Status Verified: 2024-01
• Last Update Submitted: 2024-01-04
• Last Update Posted: 2024-01-05

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 7

Inclusion Criteria

Healthy, male, endurance-trained participants who regularly run more than 40 km/week or perform endurance exercise for more than 4.5 hours/week

• Participants who have a self-reported 5-km run time of ≤23 min
• Participants who are categorized as at least "very good" based on a study by Shvartz & Reibold (Aviat Space Environ Med. 1990 Jan;61(1):3-11), in which VO2peak is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y)
• Ability to complete the 16-km familiarization run on Session 2
• Aged 18-40 years old

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Exclusion Criteria

• • Inability to meet health and physical activity guidelines according to the PAR-Q+.
• Female: hormonal fluctuations associated with the menstrual cycle have been reported to alter protein metabolism during exercise and may influence specific amino acid requirements at rest; as such, the present study will test the effect of endurance exercise on protein requirements in males only to ensure a stable hormonal environment and to increase the homogeneity of the physiological response. (NOTE: we plan to test females in a future trial based off of the results obtained herein)
• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
• Regular tobacco use
• Illicit drug use (e.g. growth hormone, testosterone, etc.)

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Nonexercised rested day	Endurance exercise	Athlete will not exercise on trial day.
Recovery from 10KM run	Endurance exercise	Athlete will run 10km on trial day.
Recovery from 20KM run	Endurance exercise	Athlete will run 20km on trial day.

Primary Outcome Measures

Name	Time	Method
Phenylalanine excretion	8 hours	Excretion of 13CO2 in the breath will be used to estimate the impact of exercise on daily protein requirements. This outcome will include the enrichment of 13CO2 in exhaled breath after the ingestion of \[13C\]phenylalanine tracer and the production of CO2 determined by indirect calorimetry at rest or after exercise.

Secondary Outcome Measures

Name	Time	Method
Phenylalanine flux	8 hours	The enrichment of \[13C\]phenylalanine in the urine will be used to estimate the flux of phenylalanine in the body at rest or after exercise.
Phenylalanine oxidation	8 hours	The oxidation of \[13C\]phenylalanine will be determined by correcting phenylalanine excretion by phenylalanine flux.

Trial Locations

Locations (1)

Goldring Centre for High Performance Sport at the University of Toronto; 🇨🇦 Toronto, Ontario, Canada