The Impact of Protein Intake on Amino Acid and Protein Metabolism, and Performance During Intensified Training
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Healthy
- Sponsor
- University of Toronto
- Enrollment
- 12
- Locations
- 1
- Primary Endpoint
- Change in 24h whole body protein balance
- Status
- Completed
- Last Updated
- 7 years ago
Overview
Brief Summary
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).
Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake.
The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.
Investigators
Daniel Moore
Associate professor
University of Toronto
Eligibility Criteria
Inclusion Criteria
- •Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week
- •Participants who are categorized at least "very good" based on a study by Shvartz \& Reibold \[40\], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y), ≥47 ml/kg/min (40-44 y), ≥44 ml/kg/min (45-50 y) according to his age,
- •Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session
- •Participants will be 18-50 years old.
- •Participants are willing to abide by the compliance rules of this study
Exclusion Criteria
- •Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone.
- •Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor
- •Regular tobacco use
- •Illicit drug use (e.g. growth hormone, testosterone, etc.)
Outcomes
Primary Outcomes
Change in 24h whole body protein balance
Time Frame: Difference between day 1 and day 4 whole body protein balance
Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using \[15N\]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels
Secondary Outcomes
- Change from baseline physical performance (5 km time trial) 5days after the beginning of training(5days after the beginning of training)
- change from baseline Physical performance (muscle strength) 5days after the beginning of training(5days after the beginning of training)
- change from baseline Physical Performance (muscle power) 5days after the beginning of training(5days after the beginning of training)