The Adipose Tissue and Sex-specific Role of Adipose-derived Extracellular Vesicles in Obesity-related Skeletal Muscle Insulin Resistance.
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 150
- 试验地点
- 1
- 主要终点
- General characterization of adipocyte- or adipose tissue-derived extracellular vesicles
研究概览
简要总结
Our society is characterized by a strong increase in the prevalence of obesity, which often causes the development of cardiovascular and metabolic diseases such as type 2 diabetes. The way(s) obesity is responsible for these diseases, is still insufficiently understood. This study therefore examines the content of human fat tissue storage location- and cell type-specific extracellular vesicles (EVs) in lean and obese individuals, and the possible connection with sex, insulin sensitivity, and the blood-brain barrier.
详细描述
This study focuses on unraveling the fundamental differences in communication via extracellular vesicles (EVs) derived from fat cells and immune cells in terms of fat tissue storage location and sex among individuals with and without obesity. The study hypothesizes that the differences in EV profiles, originating from corresponding in vitro differentiated hMADS and ex vivo isolated adult fat cells and immune cells, among individuals with and without obesity can be related to sex and/or fat tissue storage location. Additionally, the effect of the isolated EVs on the blood-brain barrier function is also investigated.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Caucasian adults with obesity that have a body mass index (BMI) of at least 35 kg/m^2 and are scheduled for their first bariatric surgery.
- •Final male/female ratio of this study is 1:1
- •Minimal knowledge of Dutch to read and understand the informed consent.
- •Individuals with obesity -
排除标准
- •Smoking and/or drug abuse
- •Cardiovascular diseases (including but not limited to implanted aids like a pacemaker or defibrillator)
- •Lung and/or kidney diseases
- •Brain and/or nerve diseases
- •Malignant diseases (e.g. cancer)
- •Individuals without obesity (lean) - inclusion criteria
- •Caucasian adults with obesity that have a body mass index (BMI) of between 18 and 24.9 kg/m^2 and are scheduled for abdominal surgery
- •Female only
- •Minimal knowledge of Dutch to read and understand the informed consent.
- •Individuals without obesity (lean) - exclusion criteria
- •Smoking and/or drug abuse
- •Cardiovascular diseases (including but not limited to implanted aids like a pacemaker or defibrillator)
- •Lung and/or kidney diseases
- •Brain and/or nerve diseases
- •Endocrine diseases like type 2 diabetes
- •Malignant diseases (e.g. cancer)
结局指标
主要结局
General characterization of adipocyte- or adipose tissue-derived extracellular vesicles
时间窗: Baseline
Concentration, size, surface markers, content and morphology of extracellular vesicles derived from isolated mature adipocytes, isolated adipose tissue stem cells or isolated from complete adipose tissue explants derived from both the subcutaneous as the visceral adipose tissue biopsies will be investigated.
General characterization of adipose tissue- and blood immune cells
时间窗: Baseline
Immune cells isolated from adipose tissue and blood will be characterized by flow cytometry.
次要结局
未报告次要终点
研究者
prof. dr. Kenneth Verboven
Prof. dr.
Hasselt University