Comparison of IL-8, FGF-1 and VEGF Levels in Pulpal Tissue, GCF and Dentinal Fluid Samples of Healthy, Reversible and Irreversible Pulpitis Diagnosed Teeth
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 51
- 试验地点
- 1
- 主要终点
- IL-8, FGF-1 and VEGF expression levels in dentinal fluid, GCF and pulp tissue samples
研究概览
简要总结
This study will include 51 patients diagnosed with healthy, reversible and irreversible pulpitis. IL-8, FGF-1 and VEGF levels will be compared using pulpal tissue, gingival crevicular fluid, dentinal fluid samples collected from the teeth of these patients. First, the DOS sample will be collected non-invasively using periopaper strips without local anesthesia. Then, the caries will be cleaned by applying local anesthesia and rubber dam to the patient and a dentinal fluid sample will be collected noninvasively using PVDF membranes. In teeth with pulp exposure due to caries, the pulp tissue that needs to be removed will not be disposed of but collected as a sample. Routine root canal treatment procedures will then continue and treatment of the teeth will be completed. The collected samples will be analyzed for IL-8, FGF-1 and VEGF levels by ELISA test.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 16 Years 至 60 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Control group:
- •Unrestored, caries-free vital teeth with healthy pulp
- •Orthodontically indicated teeth for extraction
- •wisdom teeth indicated for extraction in terms of oral hygiene
- •teeth that require root canal treatment for prosthetic purposes
- •Reversible pulpitis group:
- •Teeth that react mildly to cold, hot, sour or sweet stimuli
- •Teeth with no history of spontaneous pain
- •Teeth that respond to cold test and electric pulp test within normal limits
- •Teeth that are not sensitive to chewing or percussion
- •Irreversible pulpitis group:
- •Teeth with a history of continuous moderate or severe spontaneous pain
- •Teeth sensitive to percussion
- •Teeth without periapical pathology
排除标准
- •Patients requiring antibiotic prophylaxis.
- •Patients with diabetes or haematological diseases.
- •Patients who have used antibiotics or anti-inflammatory drugs in the last three weeks.
- •Patients who are pregnant during treatment.
- •Patients with intense plaque and tartar on their teeth, redness and bleeding in the gums.
- •Patients with severe gingivitis, generalised periodontitis or periodontal pocket depth of more than 3 mm.
- •Patients with no response to pulp sensitivity tests, presence of sinus tract, presence of periapical pathology on radiography.
- •Teeth without pulp exposure after caries removal
结局指标
主要结局
IL-8, FGF-1 and VEGF expression levels in dentinal fluid, GCF and pulp tissue samples
时间窗: Day 1
IL-8, FGF-1 and VEGF levels will be measured in dentinal fluid, GCF and pulp tissue samples obtained during routine root canal treatment. The analysis will be performed using ELISA. The aim is to compare expression levels between patients with healthy pulp, reversible pulpitis and irreversible pulpitis.
次要结局
- Correlation analysis between IL-8, FGF-1 and VEGF levels in dentinal fluid, GCF and pulp tissue samples(Day 1)
研究者
Ali Erdemir
Professor doctor
Kırıkkale University