Skeletal Muscle Protein Synthetic Response to Amino Acid and Peptides
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Fractional Synthetic Rate of Myofibrillar Proteins
研究概览
简要总结
Skeletal muscle quality is an important determinant of exercise performance and overall health. It is vital for not just movement, but also metabolizing nutrients. Protein from the diet can promote muscle protein synthesis for muscle recovery and growth. More importantly, doing so shifts net protein balance positively (e.g. protein synthesis is greater than protein breakdown) and promotes greater rates of muscle protein turnover. Leucine is an amino acid required to build muscle, but it also acts as a signaling molecule informing the muscle to start protein synthesis. Before reaching skeletal muscle, dietary protein is digested into small peptides and free amino acids. Rate of absorption from the intestine to the blood stream is significantly faster for peptides compared to amino acids. As amino acid availability in the blood is a precursor for muscle protein synthesis, our objective is to determine if the different absorption rates between free amino acid and peptides influence muscle protein synthetic and breakdown rates.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Recreationally-active adults: ≥ 30 min of physical activity at moderate intensity ≥ 3 times per week
- •Ages: 18-35 years old
- •English fluency
排除标准
- •Tobacco, nicotine (patch/gum) use (previous 6 mo)
- •Alcohol consumption >10 drinks per week
- •Metabolic disorders (e.g., Metabolic Syndrome, Diabetes, thyroid diseases)
- •Cardiovascular disease, arrhythmias
- •Hypogonadism
- •History of uncontrolled hypertension
- •Orthopedic injury/surgery (within 1 yr)
- •Hepatorenal, musculoskeletal, autoimmune, or neurological disease
- •History of neuromuscular problems
- •Previous participation in amino acid tracer studies
- •Predisposition to hypertrophic scarring or keloid formation
- •Consumption of ergogenic-levels of dietary supplements that may affect muscle mass (e.g., creatine, HMB), insulin-like substances, or anabolic/catabolic pro-hormones (e.g., DHEA) within 6 weeks prior to participation
- •Consumption of thyroid, androgenic, or other medications known to affect endocrine function
- •Consumption of medications known to affect protein metabolism (e.g., prescription-strength corticosteroids, non-steroidal anti-inflammatories, or acne medication)
- •Currently pregnant
结局指标
主要结局
Fractional Synthetic Rate of Myofibrillar Proteins
时间窗: Postabsorptive for 3.5 hours, Postprandial for 3 hours.
Myofibrillar muscle protein synthesis rates will be assessed during the 3.5hr Postabsorptive period (e.g. non-fed state) and the 3hr postprandial period (e.g. fed state) for both experimental interventions (e.g. leucine and leucine peptide). This will allow us to assess the change in fractional synthetic rate from the Postabsorptive period to the postprandial period.
Fractional Synthetic Rate of Mixed Muscle Proteins
时间窗: Postabsorptive for 3.5 hours, Postprandial for 3 hours.
Mixed muscle protein synthesis rates will be assessed during the 3.5hr Postabsorptive period (e.g. non-fed state) and the 3hr postprandial period (e.g. fed state) for both experimental interventions (e.g. leucine and leucine peptide). This will allow us to assess the change in fractional synthetic rate from the Postabsorptive period to the postprandial period.
Fractional Breakdown Rate of Mixed Muscle Proteins
时间窗: Postprandial for 1 hour
Mixed muscle protein breakdown rates will be assessed during the first hour of the postprandial period (e.g. fed state) for both experimental interventions (e.g. leucine and leucine peptide). This will allow us to assess the change in fractional breakdown rate for each respective experimental intervention.
次要结局
- Phosphorylation of muscle anabolic signaling(Baseline, immediately after ingestion of leucine/leucine peptides, and 3 hours after ingestion of leucine/leucine peptides)
研究者
Nicholas Burd
Assistant Professor
University of Illinois at Urbana-Champaign