Compared Effect of a Fruit Milk Shake With a Protein-Carbohydrate Supplement on Muscle Damage, Inflammation, Oxidative Stress and Functional Recovery, After Resistance Exercise
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 13
- 试验地点
- 2
- 主要终点
- Maximal peak torque of the knee extensors muscles
研究概览
简要总结
The aim of this study is to assess the effect of ingesting a commercial carbohydrate and protein dietary supplement in powder form (P-CHO supplement) or a milk shake with skimmed milk, strawberries and banana (MS), after resistance exercise, in muscle damage, oxidative stress, inflammation and functional recovery. It is hypothesised if the ingestion of a milk shake with skimmed milk and fruit (strawberry and banana) has the same impact on markers of muscle damage, oxidative stress, inflammation and functional recovery induced by resistance exercise, as the intake of a commercial powder with the same CHO and protein amounts.
Fifteen adult athletes from the Portuguese Athletic Federation will complete 2 trials separated at least by 2 weeks. Alternate legs and drinks will be used in each trial and participants will be overnight-fasted. This study will have a single-blind, randomized, crossover, repeated-measures experimental design. In each trial, after warm-up, the eccentric peak torque of the knee joint extensors will be determined using an isokinetic dynamometer. After this, participants will complete an isokinetic exercise until exhaustion at a constant angular velocity of 60° • s-1. After the exhaustion protocol, athletes will perform again the peak torque determination. Immediately after, participants will drink the P-CHO supplement or MS during the first 2 h. Both drinks will contain 0.8-1.2 g carbohydrates • kg-1 • h-1 and 0.2-0.4 g protein • kg-1 • h-1. Twenty four and 48 h after the exhaustion protocol participants will return to the laboratory to repeat the peak torque determination. Blood samples will be collected before warming-up, immediately and 2 h after the last peak torque determination and 24 h and 48 h after. Serum samples will be analyzed for creatine kinase, lactate dehydrogenase, interleukine-6, protein carbonyls and total antioxidant status. The delayed onset muscle soreness, using a visual analogue scale, and girths will be measured at the same moments as blood sampling. Two-way repeated-measures ANOVA will be used for statistical analysis of the data.
详细描述
Sample:
Fifteen adult athletes from the Portuguese Athletic Federation will be invited to participate in this study. They must be healthy and lactose tolerant. Athletes will be voluntary and should receive an explanatory document with the aims of the study. They must also sign an informed consent to participate. To help ensuring that the metabolic conditions are similar between trials, subjects will be instructed to fulfill a food record and a physical activity record of the 2 days prior to each trial (Shirreffs, Watson, & Maughan, 2007). It will be asked not to make drastic changes in diet and to avoid strenuous exercise during this period.
Experimental Protocol:
This study will have a single-blind, randomized, crossover, repeated-measures experimental design. As shown in Figure 1, each participant should complete 2 trials, with a wash out period of, at least, 2 weeks. In one trial subjects will randomly drink the P-CHO supplement and in the other, the MS. In order to minimize any repeated bout effect, different legs will be used in each trial (Howatson et al., 2010).
About one week before the exercise protocol, participants will be familiarized with the experimental procedures and measurements. At this time, anthropometric assessments (BW, height and body fat percentage) will also be taken.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy and lactose tolerant
排除标准
- 未提供
结局指标
主要结局
Maximal peak torque of the knee extensors muscles
时间窗: Up to 48 h
To monitor functional recovery
Creatine kinase
时间窗: Up to 48 h
To monitor muscle damage
Lactate dehydrogenase
时间窗: Up to 48 h
To monitor muscle damage
Interleukine-6
时间窗: Up to 48 h
To monitor the inflammatory process
Total antioxidant status
时间窗: Up to 48 h
To monitor oxidative stress
Delayed onset muscle soreness
时间窗: Up to 48 h
By a visual analogue scale, 10 cm in length. To monitor muscle soreness
Mid-thigh girth, Three other girths will be measured: 5, 15 and 25 cm above the superior border of the patella.
时间窗: Up to 48 h
To monitor the inflammatory process
Protein carbonyls
时间窗: Up to 48 h
To monitor oxidative stress
次要结局
- Percentage of body fat(1 week before the first trial)
- Food records(2 days before each trial)
- Physical activity records(2 days before each trial)
研究者
Monica Sousa, Principal Investigator
BSc
Universidade do Porto