In Vitro Effects of a New Chlorhexidine-based Solution on Human Gingival Fibroblasts Survival and Function
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 3
研究概览
简要总结
This prospective in vitro study aims to evaluate the effects of two chlorhexidine-based mouthwash formulations on the survival and function of human gingival fibroblasts (hGFs). Gingival fibroblasts obtained from discarded gingival tissue collected during routine dental procedures will be cultured and exposed to different concentrations of a conventional 0.2% chlorhexidine mouthwash and a 0.2% chlorhexidine mouthwash supplemented with N-acetylcysteine (NAC) and hyaluronic acid (HA).
Cell viability, apoptosis, migration, and gene expression will be assessed at multiple time points using Trypan Blue exclusion assay, fluorescence-activated cell sorting (FACS), scratch assay, and quantitative real-time PCR. The study is designed to investigate the concentration-dependent effects of chlorhexidine on fibroblast viability and wound-healing-related functions and to determine whether the addition of NAC and HA may improve the biological compatibility of chlorhexidine-based formulations.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male or female adults aged 18 years or older.
- •Undergoing routine dental surgical procedures (e.g., tooth extraction or gingivectomy).
- •Willing and able to provide written informed consent for the use of discarded gingival tissue for research purposes.
- •Systemically healthy.
排除标准
- •Presence of systemic diseases.
- •Presence of relevant medical comorbidities.
- •Current smoker.
- •Refusal to provide informed consent for the use of discarded biological material.
研究者
Rotundo Roberto
Associate Professor
IRCCS San Raffaele