Exercise Response in Humans With Obesity
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- Mayo Clinic
- 入组人数
- 120
- 试验地点
- 2
- 主要终点
- Change in muscle protein synthesis
研究概览
简要总结
This study is being done to understand how chronic inflammation affects muscle function and responses to exercise.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Obese men and women ≥ 20 and ≤ 45 years of age.
排除标准
- •Diabetes or fasting plasma glucose >126 mg/dL
- •Anemia (female subjects hemoglobin of <11 g/dl and male subjects hemoglobin <12 g/dl)
- •Active coronary artery disease or history of unstable macrovascular disease (unstable angina, myocardial infarction, stroke, and revascularization of coronary, peripheral or carotid artery within 3 months of recruitment)
- •Renal failure (serum creatinine > 1.5mg/dl)
- •Chronic active liver disease defined as aspartate aminotransferase (AST)>144 IU/L or alanine transaminase (ALT) >165 IU/L)
- •Oral warfarin group medications or history of blood clotting disorders.
- •Pregnancy or breastfeeding
- •Alcohol consumption greater than 2 glasses/day or other substance abuse
- •Untreated or uncontrolled hypothyroidism
- •Debilitating chronic disease (at the discretion of the investigators)
研究组 & 干预措施
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Treadmill Test (Diagnostic Test)
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Strength Test (Diagnostic Test)
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Dual-Energy X-Ray Absorptiometry (Diagnostic Test)
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Muscle Biopsy (Procedure)
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Fat Biopsy (Procedure)
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Resting Energy Expenditure (Diagnostic Test)
Lean Group
Participants with body mass index 20-25 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Magnetic resonance imaging (Diagnostic Test)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Treadmill Test (Diagnostic Test)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Strength Test (Diagnostic Test)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Dual-Energy X-Ray Absorptiometry (Diagnostic Test)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Muscle Biopsy (Procedure)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Fat Biopsy (Procedure)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Resting Energy Expenditure (Diagnostic Test)
Obese Group
Participants with body mass index 30-45 kg/m^2 will complete an outpatient study visit (body composition, blood draw, treadmill test, strength test) and an inpatient visit (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
干预措施: Magnetic resonance imaging (Diagnostic Test)
结局指标
主要结局
Change in muscle protein synthesis
时间窗: Baseline, post-exercise approximately 8 hours
Skeletal muscle protein synthesis will be measured from the rate of incorporation of infused amino acids measured by mass spectrometry.
次要结局
- Change in mRNA expression of exercise responsive genes(Baseline, post-exercise approximately 8 hours)
- Change in expression and activation (phosphorylation) of signaling proteins(Baseline, post-exercise approximately 8 hours)