The Relationship of Adiponectin in Adipose Tissue, Thy-1 in Plaques, and Inflammatory
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 96
- 试验地点
- 1
- 主要终点
- CD91 (Thy-1)
研究概览
简要总结
Atherosclerosis is an inflammatory process in which immune mechanisms interact with metabolic risk factors to initiate, propagate, and activate lesions in the arterial trees and is known as the main cause of coronary artery disease (CAD). Recently, there are more and more studies highlighted the potential importance of adipose tissue in relation to inflammation effects on CAD pathogenesis. However, it remains unclear whether Thy-1, adiponectin or any other inflammatory mediators in mediastinal adipose tissue contribute to CAD. In this study, we aim to analyze the expression of inflammatory mediators' expression via in vitro assay (3T3-L1 cell culture) and in vivo assay (human adipose tissues).
详细描述
In addition, we'd like to investigate the association of the investigators' findings with clinical atherosclerotic risks, medications (statins or antiplatelet), and blood sugar. Now, we've established cell assay and successfully transformed mature adipocyte from 3T3-L1 cells. Besides, there were nighty-six patients enrolled into this study. Flow cytometry of Thy-1 and MCP-1 concentration in cultured medium by ELISA were also analyzed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •coronary artery bypass grafting surgery
- •valvular surgery
排除标准
- •liver disease (GPT 2 times greater than normal limits)
- •chronic renal insufficiency (Creatinine > 2.0 mg/dL)
- •neoplastic diseases
- •taking steroids
- •congestive heart failure
研究组 & 干预措施
CAD
Patients with valve diseases proposed to have cardiac operations will be enrolled in this study, but those with congestive heart failure are excluded. Besides, those patients with valve diseases should have patent coronary arteries on coronary angiography.
干预措施: CAD (Procedure)
VHD
Patients with valve diseases proposed to have cardiac operations will be enrolled in this study, but those with congestive heart failure are excluded. Besides, those patients with valve diseases should have patent coronary arteries on coronary angiography.
干预措施: VHD (Procedure)
结局指标
主要结局
CD91 (Thy-1)
时间窗: up to 24 months
次要结局
- CD45(up to 24 months)
研究者
Shu-Hsun Chu
President
Far Eastern Memorial Hospital