Antibiogram and prevalence of Gram negative bacteria in subjects with different stages of periodontitis : a clinico-microbiological study
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- 1. Prevalence of Gram -negative bacteria in subgingival samples of patients with different stages of periodontal disease
研究概览
简要总结
This will be a cross sectional, observational study to estimate the prevalence and determine the antimicrobial susceptibility of Gram- negative bacteria from subgingival clinical isolates of patients with different stages of periodontitis. The study will be conducted among the subjects selected from the outpatient Department of Periodontics and Implantology, Mar Baselios Dental College, Kothamangalam. A total of 80 patients will be recruited (20 per group). The clinical parameters namely Plaque Index (PI), Gingival Index (GI), Probing Pocket depth (PPD) and Clinical Attachment Levels (CAL) will be assessed and the patients will be divided into different stages of periodontitis according to 2017 AAP classification. Samples will be collected from the gingival sulcus of selected teeth (after removal of supragingival calculus) using curettes and will be transferred immediately into BHI broth. The broth will immediately be sent to microbiology lab for further evaluation.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 30.00 Year(s) 至 60.00 Year(s)（—）
- 性别
- All
入选标准
- •Patients diagnosed with periodontitis as per 2017 AAP classification
- •Systemically healthy individuals
- •Patient with age group from 30-60yrs
- •Patients who are willing to participate in the study.
排除标准
- •Patients who have undergone any periodontal therapy in past 6 months.
- •Patients undergoing orthodontic treatment
- •Patients with systemic diseases (e.g. diabetes, hypertension)
- •Patients who are edentulous.
- •Use of topical or systemic antimicrobials in the last 6 months
- •Use of anti-inflammatory drugs in the last 3 months
- •Smoking or use of smokeless tobacco.
结局指标
主要结局
1. Prevalence of Gram -negative bacteria in subgingival samples of patients with different stages of periodontal disease
时间窗: At initial visit
2. Correlate prevalence of different Gram-negative bacteria with stages of periodontitis in the above patients
时间窗: At initial visit
3. Determine antimicrobial susceptibility of Gram -negative bacteria from above samples
时间窗: At initial visit
次要结局
未报告次要终点
研究者
Anu Mathew
Mar Baselios Dental College