Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 45
- 试验地点
- 2
- 主要终点
- mRNA expression of adipose tissue metabolic enzymes
研究概览
简要总结
The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age:18-40
- •Body Mass Index: 20-30 kg/m2
- •Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
- •Women must have regularly occurring menses and must be premenopausal
- •Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository
排除标准
- •Evidence/history of cardiovascular or metabolic disease.
- •Taking anti-coagulate medications or medications known to affect metabolism or inflammation
- •Weight instability ≥ ±3kg in the last 6 months
- •Tobacco or e-cigarette users
- •Women must not be pregnant or actively lactating
结局指标
主要结局
mRNA expression of adipose tissue metabolic enzymes
时间窗: 75 minutes after exercise
qPCR
mRNA expression of skeletal muscle oxidative enzymes
时间窗: 60 minutes after exercise
qPCR
post-translational modifications of skeletal muscle oxidative enzymes
时间窗: 60 minutes after exercise
Western blot
post-translational modifications of Adipose tissue metabolic proteins
时间窗: 75 minutes after exercise
Western blot
次要结局
未报告次要终点
研究者
Jeffrey F Horowitz
Professor of Movement Science and Director of the Substrate Metabolism Laboratory (SML) at the University of Michigan School of Kinesiology
University of Michigan