Impact of Short-term Overfeeding With a Saturated Fat-rich Diet on Fasted Systemic and White Adipose Tissue Inflammatory Responses
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 1
- 主要终点
- Protein expression (content and phosphorylation) of key markers of metabolic inflammation in white adipose tissue (for example assessment of NFKB/IKBa total protein and phosphorylation by western blot analysis)
研究概览
简要总结
This study will investigate the effect of a 7-day westernised high-fat (65% of kilocalories), high-calorie (150% of requirements) diet on markers of inflammation in the blood and white adipose tissue. Participants will firstly complete a 3-day weight maintenance phase (Days 1-3) before completing a 7-day high fat diet intervention (Days 4-10). On days 4 and 11 participants will complete a laboratory visit where anthropometric measurements, blood and adipose samples will be collected.
The investigators hypothesise that consuming a high-fat, high-calorie diet for 7 days will alter the inflammatory responses in white adipose tissue and will induce metabolic endotoxaemia / systemic inflammation.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •18-40 years
- •BMI = 21.0-29.9 kg/m2
- •Male or female
- •Physically active (> 3 x 30 min moderate exercise per week)
- •No cardiometabolic (e.g. heart disease, high blood pressure, type 2 diabetes) or inflammatory illness
排除标准
- •Inactive (< 3 x 30 min moderate exercise per week)
- •Women who are pregnant or lactating
- •Medication/supplements known to interfere with study outcomes or prescribed antibiotics within the last 3 months
- •Unstable weight history (≥3kg loss or gain in the previous 3 months)
- •An allergy to lidocaine
- •Food allergies (e.g. gluten, dairy) and intolerances (e.g. lactose) which could impede compliance to the diet
- •Vegetarian/ vegan (as the high-fat diet is based on animal fats)
- •Consumption of probiotics yogurts during or within the four weeks prior to the start of the study (e.g. Actimel, Activia, Yakult, Yeo Valley)
- •Alcohol consumption >28 units per week for a man (i.e. not more than 14 pints of beer or 28 small glasses of wine) or >21 units per week for a woman (i.e. more than 10 and a half pints of beer or 21 small glasses of wine)
- •Any other unusual medical history or diet and lifestyle habits or practices that would preclude volunteers from participating in a dietary intervention or metabolic study
- •Restrained eaters (determined by Three Factor Eating Questionnaire)
研究组 & 干预措施
High Fat Diet
Participants will consume a high-fat, high-calorie diet for 7 days (i.e. westernised diet) following a 3-day weight maintenance diet. Measurements will be made pre- and post-high fat diet intervention.
干预措施: High Fat Diet (Other)
结局指标
主要结局
Protein expression (content and phosphorylation) of key markers of metabolic inflammation in white adipose tissue (for example assessment of NFKB/IKBa total protein and phosphorylation by western blot analysis)
时间窗: Change between pre- and post-high fat diet (assessed on days 4 and 11)
This will be assessed following the collection of fasted white adipose tissue samples
次要结局
- Immune Cell Populations in the Blood(Change between pre- and post-high fat diet (assessed on days 4 and 11))
- Gene expression of key markers of metabolic inflammation in white adipose tissue(Change between pre- and post-high fat diet (assessed on days 4 and 11))
- Anthropometric Measurements (for example height and weight that will be aggregated to report BMI in kg/m^2)(Change between pre- and post-high fat diet (assessed on days 4 and 11))
- Physical Activity(Habitual physical activity will be assessed for 3 days prior to commencing the high-fat diet intervention (days 1-3) and during the final 3 days of the intervention (days 8-10))
- Systemic Markers of Inflammation (for example CRP, TNFa and IL-6 concentrations, determined by spectrophotometric assay/ ELISA)(Change between pre- and post-high fat diet (assessed on days 4 and 11))
- Fasting Serum Markers of Insulin Resistance (for example insulin and glucose concentrations, determined using an ELISA/ spectrophotometric assay)(Change between pre- and post-high fat diet (assessed on days 4 and 11))
- Fasting Serum Lipid Profile (for example total, HDL and LDL cholesterol, TAG and NEFA concentrations, measured using a spectrophotometric assay)(Change between pre- and post-high fat diet (assessed on days 4 and 11))
- Systemic Markers of Metabolic Endotoxemia (for example LBP and sCD14 concentrations, determined using an ELISA)(Change between pre- and post-high fat diet (assessed on days 4 and 11))
研究者
Dr Oonagh Markey
Principal Investigator
Loughborough University