Impatto Dell'Ambiente Urbano Sull'età Biologica: Effetto Della qualità Dell'Aria e Dello Stress Lavorativo a Pavia su Citochine Infiammatorie e Profilo di Metilazione Del DNA
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 151
- 试验地点
- 1
- 主要终点
- DNA Methylation Profile Associated With Urban Air Pollution Exposure
研究概览
简要总结
The goal of this observational study is to evaluate the impact of the urban exposome, including air pollution and lifestyle-related stressors, on biological aging, inflammatory pathways, oxidative stress, and DNA methylation patterns in healthy workers exposed to urban air pollution in the city of Pavia, Italy.
The study population included 151 adult workers, divided into two groups: outdoor workers (including police officers, taxi drivers, and dustmen) and an indoor control group composed of office workers from the University of Pavia and ICS Maugeri hospital with minimal outdoor exposure.
The main questions it aims to answer are:
- Does chronic exposure to urban air pollutants such as NO2, SO2, O3, benzene, volatile organic compounds, and PM2.5 influence inflammatory cytokines, oxidative stress markers, and biological aging?
- Is exposure to urban pollution associated with changes in DNA methylation patterns and epigenetic age acceleration?
- How do lifestyle factors, nutritional status, sleep quality, physical activity, smoking habits, and perceived quality of life interact with the urban exposome and biological outcomes?
Researchers will compare outdoor workers with indoor workers to evaluate differences in environmental exposure, inflammatory biomarkers, oxidative stress, DNA methylation profiles, and estimated biological age.
Participants will:
- Undergo clinical assessments, with interviews regarding occupational history.
- Undergo anthropometric and nutritional evaluations, including body composition analysis and blood chemistry tests; answer questionnaires about physical activity, smoking habits, sleep quality, and perceived quality of life; undergo interviews about dietary habits.
- Wear passive personal air samplers for approximately 5 working days to measure exposure to urban air pollutants.
- Provide blood samples for analysis of inflammatory cytokines, oxidative stress markers, thyroid-related biomarkers, and epigenetic modifications.
- Undergo DNA methylation analyses using epigenetic techniques to estimate biological age and evaluate associations between pollutant exposure and epigenetic changes.
- Receive feedback regarding their environmental exposure and general health and nutritional status.
This interdisciplinary observational study adopts an exposome-based approach integrating environmental monitoring, occupational medicine, nutrition, and epigenetics to better understand the relationship between urban environmental exposure and human health.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adults aged 18 years or older;
- •Male or female participants;
- •Ability and willingness to provide written informed consent;
- •Employment in occupations performed predominantly outdoors in an urban environment with exposure to atmospheric pollution, i.e. employment as urban police officers, police personnel, public transport workers, taxi drivers, newsstand workers, or other outdoor urban workers in the city of Pavia;
- •Employment in predominantly indoor office-based occupations with minimal occupational exposure to outdoor urban air pollution (control group), i.e. workers undergoing occupational health surveillance at ICS Maugeri or affiliated institutions.
排除标准
- •Current diagnosis of active oncological disease;
- •Current treatment with immunosuppressive therapy;
- •Inability or unwillingness to provide informed consent;
- •Medical conditions preventing participation in blood sampling or study assessments;
- •Pregnancy or breastfeeding (if applicable and according to Ethics Committee recommendations).
研究组 & 干预措施
Outdoor exposed workers
The group consists of outdoor workers, specifically traffic police, taxi drivers and dustmen working in Pavia city area (Italy). The group is highly exposed to urban air pollutants, therefore these urban workers are particularly vulnerable to biological effects such as genetic and hormonal alterations, oxidative stress, and inflammation, which could predispone to chronic non-communicable diseases.
The exposome variables are compared between the Outdoor exposed workers group and the Indoor non-exposed workers group, to identify differences among the group considering biological clock, inflammatory status and oxidative stress as the main outcome.
Indoor non exposed workers
The group consists of indoor workers, specifically administrative staff of Maugeri Clinic and office workers from the University of Pavia. They are minimally exposed to urban pollutants, therefore represent the "control" group of the study.
结局指标
主要结局
DNA Methylation Profile Associated With Urban Air Pollution Exposure
时间窗: Baseline
DNA methylation patterns are evaluated in peripheral blood samples from participants exposed to different levels of urban air pollution. Genomic DNA is extracted and analyzed using bisulfite sequencing, quantitative polymerase chain reaction (qPCR), and pyrosequencing techniques to quantify DNA methylation in specific genomic regions. Methylation measures are evaluated in relation to participants' measured exposure to urban air pollutants.
次要结局
- Biological Age Estimated using an Epigenetic Clock Analysis in Workers Exposed to Urban Air Pollution(Baseline)