Regulation of Lipolysis by Insulin in Skeletal Muscle and Adipose Tissue in Type 2 Diabetes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Change from baseline in insulin sensitivity of adipose tissue and skeletal muscle lipolysis
研究概览
简要总结
Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 45 Years 至 65 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Caucasian
- •Normal blood pressure (SBP 100-140 mmHg, DBP 60-90 mmHg)
- •weight stable in last 3 months
排除标准
- •people with intensive fitness training (e.g. athletes > 3 times/week)
- •History of cardiovascular diseases
- •Bleeding disorders
- •Use of medication interfering with the study endpoints/hypotheses (e.g. beta-blockers)
- •Not to be able to understand the study information
- •Subjects on a special diet or vegetarian
- •Blood donation 2 months prior to the study and during the study
- •Participating in an other study
结局指标
主要结局
Change from baseline in insulin sensitivity of adipose tissue and skeletal muscle lipolysis
时间窗: 8, 20 and 40 mU insulin (2 hours)
Changes from baseline in glycerol (lactate, pyruvate and glucose) in tissue microdialysate during a 3 step euglycemic hyperinsulinemic clamp (8, 20 and 40mU of insulin), every step for 2h
次要结局
- Baseline skeletal muscle lipid accumulation(Baseline)
- Baseline adipose tissue and skeletal muscle gene expression(baseline)