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Skeletal Muscle Protein Metabolism and Insulin Sensitivity in Overweight Individuals: Effects of Meals With Various Fatty Acid Compositions

Not Applicable
Completed
Conditions
Insulin Resistance
Registration Number
NCT03146286
Lead Sponsor
University of Nottingham
Brief Summary

The aim of the study will investigate whether impairment in the action of insulin to promote the use of glucose in skeletal muscle (insulin resistance) as a result of oral ingestion of a liquid meal rich in saturated fat is linked to reduced ability of muscles to synthesise new protein in response to dietary protein intake, which ultimately may compromise maintenance of muscle size and quality of life and whether partially replacing saturated fat in the liquid meal with omega 3 polyunsaturated FA (n3PUFA) will ameliorate these negative effects.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
8
Inclusion Criteria
  • Male, healthy, overweight (BMI>27 kg/m2 and fat mass >30%) non-diabetic individuals.
Exclusion Criteria
  • Diabetes, smoking, cardiovascular disease

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Change in muscle protein fractional synthetic rate3 hours post protein drink

Measurement of FSR at baseline and post intervention

Secondary Outcome Measures
NameTimeMethod
whole body postprandial insulin sensitivityBaseline and every 15mins for 7 hours

Glucose and insulin area under the curves (AUC) in response to the milk protein and glucose ingestion will be measured

Whole body and skeletal muscle fat oxidationBaseline and every 15mins for 7 hours

D31palmitate plasma enrichment and incorporation into skeletal muscle lipids

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