The Effect of an Acute Maximal Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 86
- 试验地点
- 1
- 主要终点
- Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
研究概览
简要总结
This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect.
Therefore, this study hypothesizes that:
H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise.
H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism.
H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels.
H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism.
H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Athletes group:
- •Must be basketball, or volleyball, or handball players.
- •Must be subjects who have been training regularly for at least 3 months.
- •Sedentary group:
- •* Must be subjects who have not been training regularly for at least 3 months.
- •For both groups:
- •Non obese (body mass index < 30).
- •Aged between 18 and 35 years.
排除标准
- •For both groups:
- •Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances.
- •Being obese (body mass index > 30).
- •Having an acute or chronic injury which is limiting physical performance.
- •Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples.
- •Being injured or sick during the study.
结局指标
主要结局
Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
时间窗: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory.
Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
时间窗: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory.
Uncoupling protein-1-3826 A/G polymorphism determination
时间窗: 15 minutes before the Yo-Yo intermittent recovery test (level 1)
Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory.
次要结局
- Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)(15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1))
- Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)(15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1))
研究者
Jeffry Roy Chauchat
Senior instructor
Eastern Mediterranean University