Effects of Exercise on Fructose Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 8
- 试验地点
- 2
- 主要终点
- Plasma triglyceride
研究概览
简要总结
A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate
- how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals
- how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •18-40 years old
- •19 kg/m2>BMI>25kg/m2
- •moderate to high usual physical activity
- •non-smokers
排除标准
- •family history of diabetes mellitus
- •ECG anomaly
- •any medication
- •participation to blood spending or other clinical study in the 3 months before the beginning of this study
- •consumption of drugs
- •consumption of more than 50g alcool/week
- •family history of food intolerance
结局指标
主要结局
Plasma triglyceride
时间窗: measurements in fasting conditions and every hour from 7:00 am to 4:00 pm (up to 9 hours)
measurement of total and VLDL-TG concentrations in study 1
intramyocellular lipids
时间窗: after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise
Measurement by 1H-MRS in muscle vastus lateralis in study 2
Fructose conversion into lipids
时间窗: measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)
Estimated from 13C palmitate-VLDL concentration
次要结局
- Fructose conversion into glucose(measured between 7:00 am and 4:oo pm (up to 9 hours))
- intramyocellular glycogen concentrations(after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise)
- fructose oxidation(measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours))
- exogenous lipid oxidation(measured from 7:00 am to 4:00 pm in study 2 (up to 9 hours))
研究者
Luc Tappy, MD
Professor of Physiology
University of Lausanne