Looking for a Blood Epigenetic Signature to Predict Female Infertility
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 1,456
- 试验地点
- 1
- 主要终点
- differences of DNA methylationin the peripheral venous blood of new patients undergoing ART
研究概览
简要总结
The present research project aims to study the DNAm mechanisms underlying the reduction of fertility due to the progressive depletion of oocyte quality. Specifically, our project aims to build an epigenetic clock for MGCs by using outcomes that are certainly related to female fertility. The validation of such findings will be carried out on peripheral blood in order to guarantee its non-invasiveness and allow for any clinical transferability. In order to identify a blood epigenetic signature able to predict female infertility, we planned to explore the problem from different points of view by conducting several studies in different settings.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 43 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •18< age <43, Infertile woman undergoing ART, recurrent pregnancy loss
排除标准
- •age < 18 or > 44, previous ovarian surgery, severe male factor infertility
结局指标
主要结局
differences of DNA methylationin the peripheral venous blood of new patients undergoing ART
时间窗: 3 years
The specific epigenetic modification will be searched in the peripheral venous blood of two new cohorts of patients referred to PMA centres.
differences of DNA methylationin the peripheral venous blood of patients undergoing ART
时间窗: 3 years
search for this methylation difference found in the granulosa cell sample, also in the peripheral venous blood of these same patients
differences of DNA methylation in granulosa cells of patients undergoing ART
时间窗: 3 years
The epigenetic profile of DNA extracted from granulosa cells of patients undergoing ART (COHORT1) will be examined to identify position and region's differences of DNA methylation in pregnant women (fertile women) and in who does not get pregnant (infertile women)
impact of exogenous pro-inflammatory factors
时间窗: 3 years
on all the above cohorts will be investigated, via online questionnaire
differences of DNA methylationin the peripheral venous blood of women attempting to conceive naturally
时间窗: 3 years
The specific epigenetic modification will be searched in the peripheral venous blood of women attempting to conceive naturally (COHORT2)
次要结局
未报告次要终点
研究者
Valeria Stella Vanni
Doctor
Università Vita-Salute San Raffaele