Oocyte Quality Assessment Using Spectrophotometer
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 200
- 试验地点
- 1
- 主要终点
- Difference in follicular fluid infrared spectrophotometric profiles between high-quality and low-quality oocytes.
研究概览
简要总结
The aim of the study is to check whether follicular fluid evaluation using a spectrophotometer can differentiate high quality oocytes from low quality oocytes. Oocyte quality will be defined using GDF9, BMP15 and oocyte morphology. The correlation between cortisol levels in women and oocyte quality parameters will be estimated.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Screening
- 盲法
- None
入排标准
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Women aged 20-40 years.
- •Undergoing IVF or IVF-ICSI treatment.
- •At least five follicles visualized on ultrasound at the time of oocyte retrieval
- •Ability to provide written informed consent.
排除标准
- •Age >40 years.
- •Fewer than five follicles at the time of oocyte retrieval.
- •Use of donor oocytes.
- •Significant medical comorbidities affecting ovarian function.
- •Known ovarian malignancy or prior ovarian surgery significantly affecting follicular integrity.
结局指标
主要结局
Difference in follicular fluid infrared spectrophotometric profiles between high-quality and low-quality oocytes.
时间窗: 3 Years
Infrared spectrophotometric measurements of follicular fluid collected at oocyte retrieval will be obtained using attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR). Oocytes will be classified as high-quality or low-quality based on predefined morphological criteria and follicular fluid levels of GDF9 and BMP15 measured by ELISA. The primary outcome will be reported as differences in normalized spectral absorbance features between the two oocyte quality groups, including discrimination performance using multivariate analysis.
次要结局
未报告次要终点